The effect of ethanol fixation on stable isotope signatures in benthic organisms
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概要
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Stable isotope analyses are often used for the estimation of food web structures based on empirical fractionation (i.e., enrichment of carbon isotope signatures at 0–1‰ with one trophic level, and that of nitrogen at 3–4‰). We examined the effect of 80% ethanol fixation on both carbon and nitrogen stable isotope signatures of six species of macrobenthos samples. The effect of fixation was inconsistent (i.e. both enrichment and depletion occurred). The shift of nitrogen isotope signatures due to fixation ranged from −0.3 to +0.8‰, and the carbon signatures of fixed samples showed enrichment by −0.3 to +1.3‰ depending on the species. These changes would clearly affect estimation of trophic levels and energy flow in food webs. Ethanol fixation may not be a smart choice because of the inconsistency of the effect on isotope signatures. However, if the effect was corrected for each species, ethanol-fixed samples can be practically useful for food web analyses using stable isotopes.
- 2010-05-01
著者
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YOSHINO KENJI
Ariake Sea Research Project, Saga University
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AMANO YOSHIMASA
Ariake Sea Research Project, Saga University
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FUKUMORI KAYOKO
Center for Marine Environmental Studies, Ehime University
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HAMAOKA HIDEKI
Center for Marine Environmental Studies, Ehime University
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MIYASAKA HITOSHI
Center for Marine Environmental Studies, Ehime University
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OMORI KOJI
Center for Marine Environmental Studies, Ehime University
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Fukumori Kayoko
Ehime Univ. Mastuyama Jpn
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Fukumori Kayoko
Center For Marine Environmental Studies Ehime University
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Yoshino Kenji
Ariake Sea Research Project Saga University
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OHMORI KOJI
Center for Marine Environmental Studies, Ehime University
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Miyasaka Hitoshi
Center For Marine Environmental Studies Ehime University
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Miyasaka Hitosihi
The Kansai Electric Power Co. Environmental Research Center
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Miyasaka Hitoshi
Kansai Electric Power Co. Technical Research Center Environmental Research Center:rite Amagasakti 2n
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Omori Koji
Center For Marine Environmental Studies Ehime University
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Omori Koji
Center For Marine Environmental Studies (cmes) Ehime University
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Hamaoka Hideki
Center For Marine Environmental Studies Ehime Univ.
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Hamaoka Hideki
Center For Marine Environmental Studies Ehime University
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Miyasaka Hitoshi
Department Of Chemistry Faculty Of Science Tokyo Metropolitan University
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Amano Yoshimasa
Ariake Sea Research Project Saga University
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Miyasaka Hitoshi
The Kansai Electric Power Co. Environmental Res. Center Keihanna-plaza 12f 1-7 Seikacho Sourakugun K
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Miyasaka Hitoshi
Center For Ecological Research Kyoto University
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