Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages.

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概要

• 論文の詳細を見る

• Cathepsin E is an endo-lysosomal aspartic proteinase exclusively present in immune system cells. Previous studies have shown that cathepsin E-deficient (CatE(-/-)) mice display aberrant immune responses such as atopic dermatitis and higher susceptibility to bacterial infection. However, the mechanisms underlying abnormal immune responses induced by cathepsin E deficiency are still unclear. In this study, we found that the cell-surface levels of chemotactic receptors, including chemokine receptor (CCR)-2 and N-formyl peptide receptors (FPRs), were clearly diminished in CatE(-/-)macrophages compared with those in wild-type cells. Consistently, chemotaxis of CatE(-/-)macrophages to MCP-1 and N-formyl-methionyl-leucyl-phenylalanine was also decreased. Similar to the chemotactic receptors, the surface expressions of the adhesion receptors CD18 (integrin beta(2)) and CD 29 (integrin beta(1)) in CatE(-/-) macrophages were significantly decreased, thereby reducing cell attachment of CatE(-/-) macrophages. These results indicate that the defects in chemotaxis and cell adhesion are likely to be involved in the imperfect function of CatE(-/-)macrophages.

• The Japanese Biochemical Societyの論文
• 2009-05-01

著者

• 中山 浩次

  長崎大・院・医歯薬・口腔病原微生物

• 岡本 研

  日本医科大学大学院医科生物化学分野

• 中山 浩次

  長崎大・歯・口腔細菌

• 中山 浩次

  長崎大院・医歯薬・口腔病原微生物

• 岡本 研

  日本医科大学生化学分子生物学講座(構造生物学・代謝学)

• Nakayama K

  Department Of Molecular And Cellular Biology Medical Institute Of Bioregulation Kyushu University

• 中山 浩次

  長崎大学 大学院医歯薬学総合研究科歯学系口腔病原微生物学分野

• 中山 浩次

  九州大・歯・細菌

• Yamamoto K

  Proteolysis Research Laboratory Graduate School Of Pharmaceutical Sciences Kyushu University

• 中山 浩次

  長崎大学歯学部口腔細菌学講座

• OKAMOTO Kuniaki

  Department of Pharmacology, Nagasaki University School of Dentistry

• Yamamoto Kenji

  Proteolysis Research Laboratory Graduate School Of Pharmaceutical Sciences Kyushu University

• Nakayama Koji

  長崎大学 医歯薬学総合研究科口腔病原微生物学分野(口腔細菌学)

• TSUKUBA Takayuki

  Department of Pharmacology,Kyushu University Faculty of Dentistry

• YANAGAWA Michiyo

  Department of Fixed Prosthodontics, Graduate School of Dental Science, Kyushu University

• OKAMOTO Yoshiko

  Department of Biochemistry, Daiichi University College of Pharmaceutical Sciences

• YASUDA Yoshiyuki

  Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Denti

• NAKAYAMA Keiichi

  Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University

• KADOWAKI Tomoko

  Pharmacology, Graduate School of Dental Science, Kyushu University

• Okamoto Ken-ichi

  Department Of Advanced Materials Science And Engineering Yamaguchi University

• Okamoto Ken-ichi

  Graduate School Of Pure And Applied Sciences University Of Tsukuba

• 中山 浩次

  長崎大 院 医歯薬総合発生分化機能再建学講座

• Yamamoto K

  Department Of Pharmacology Graduate School Of Dental Science Kyushu University

• Kato Yuzo

  Division Of Oral Molecular Pharmacology Department Of Developmental And Reconstructive Medicine Naga

• Kadowaki T

  Pharmacology Graduate School Of Dental Science Kyushu University

• Okamoto Yoshiko

  Department Of Anatomic Pathology Graduate School Of Medical Sciences Kyushu University

• Yasuda Yoshiyuki

  Division Of Clinical Cariology And Endodontology Department Of Oral Rehabilitation School Of Dentist

• Yanagawa Michiyo

  Department Of Fixed Prosthodontics Graduate School Of Dental Science Kyushu University

• Nakayama Keiichi

  Department Of Molecular And Cellular Biology Medical Institute Of Bioregulation Kyushu University

• Tsukuba Takayuki

  Department Of Dental Pharmacology Graduate School Of Biomedical Sciences Nagasaki University

• Okamoto Ken-ichi

  Department Of Chemistry Graduate School Of Pure And Applied Sciences University Of Tsukuba

• Okamoto Ken-ichi

  Department Of Advanced Materials Science And Engineering Faculty Of Engineering Yamaguchi University

• Nakayama Koji

  Division Of Microbiology And Oral Infection Department Of Developmental And Reconstructive Medicine

• Okamoto Kouji

  Department Of Biochemical Engineering And Science Kyusyu Institute Of Technology

• Yamamoto Kenji

  Proteolysis Research Laboratory Graduate School Of Pharmaceutical Sciences Graduate School Of Dental

• Okamoto Ken

  Department Of Biochemistry And Molecular Biology Nippon Medical School

• Okamoto Kuniaki

  Department of Dental Pharmacology, Graduate School of Biomedical Sciences, Nagasaki University

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