Rhoptry neck protein RON2 forms a complex with microneme protein AMA1 in Plasmodium falciparum merozoites.
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概要
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Erythrocyte invasion is an essential step in the establishment of host infection by malaria parasites, and is a major target of intervention strategies that attempt to control the disease. Recent proteome analysis of the closely-related apicomplexan parasite, Toxoplasma gondii, revealed a panel of novel proteins (RONs) located at the neck portion of the rhoptries. Three of these proteins, RON2, RON4, and RON5 have been shown to form a complex with the microneme protein Apical Membrane Protein 1 (AMA1). This complex, termed the Moving Junction complex, localizes at the interface of the parasite and the host cell during the invasion process. Here we characterized a RON2 ortholog in Plasmodium falciparum. PfRON2 transcription peaked at the mature schizont stage and was expressed at the neck portion of the rhoptry in the merozoite. Co-immunoprecipitation of PfRON2, PfRON4 and PfAMA1 indicated that the complex formation is conserved between T. gondii and P. falciparum, suggesting that co-operative function of the rhoptry and microneme proteins is a common mechanism in apicomplexan parasites during host cell invasion. PfRON2 possesses a region displaying homology with the rhoptry body protein PfRhopH1/Clag, a component of the RhopH complex. However, here we present co-immunoprecipitation studies which suggest that PfRON2 is not a component of the RhopH complex and has an independent role. Nucleotide polymorphism analysis suggested that PfRON2 was under diversifying selective pressure. This evidence suggests that RON2 appears to have a fundamental role in host cell invasion by apicomplexan parasites, and is a potential target for malaria intervention strategies.
- 2009-03-01
著者
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CAO Jun
Department of Molecular Parasitology, Ehime University Graduate School of Medicine
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KANEKO Osamu
Department of Molecular Parasitology, Ehime University Graduate School of Medicine
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THONGKUKIATKUL Amporn
Department of Biology, Faculty of Science, Burapha University
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TACHIBANA Mayumi
Department of Molecular Parasitology, Ehime University Graduate School of Medicine
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OTSUKI Hitoshi
Department of Molecular Parasitology, Ehime University Graduate School of Medicine
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GAO Qi
Malaria Department, Jiangsu Institute of Parasitic Diseases
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TSUBOI Takafumi
Cell-Free Science and Technology Research Center, Ehime University
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TORII Motomi
Department of Molecular Parasitology, Ehime University Graduate School of Medicine
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Cao Jun
Department Of Molecular Parasitology Ehime University Graduate School Of Medicine
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Gao Qi
Malaria Department Jiangsu Institute Of Parasitic Diseases
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Otsuki Hitoshi
Department Of Molecular Parasitology Ehime University Graduate School Of Medicine
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Tachibana Mayumi
Department Of Molecular Parasitology Ehime University Graduate School Of Medicine
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Tsuboi T
Cell-free Science And Technology Research Center Ehime University
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Tsuboi Takafumi
Cell-free Science And Technology Research Center Ehime University
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Torii M
Department Of Molecular Parasitology Ehime University Graduate School Of Medicine
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Thongkukiatkul Amporn
Department Of Biology Faculty Of Science Burapha University
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Cao Jun
Department Of Chemistry Faculty Of Science Saitama University
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Kaneko Osamu
Department Of Molecular Parasitology Ehime University Graduate School Of Medicine
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Torii Motomi
Department Of Molecular Parasitology Ehime University Graduate School Of Medicine
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