Bone Morphogenetic Protein-2 (BMP-2) and Vascular Endothelial Growth Factor (VEGF) Transfection to Human Periosteal Cells Enhances Osteoblast Differentiation and Bone Formation

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概要

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• Periosteum has been demonstrated to contain mesenchymal progenitor cells differentiating to osteoblasts, and both bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) may play important roles in cell-based approaches to bone regeneration. The purpose of this study was to evaluate the feasibility and efficacy of BMP-2 and/or VEGF on periosteal cell differentiation to osteoblasts in vitro and ectopic bone formation in vivo. Human periosteum-derived cells were transfected with BMP-2, VEGF, BMP-2 + VEGF, or vehicle as a control by non-viral gene transfer and then cultured and implanted to nude mice intramuscularly. Real-time polymerase chain reaction analysis of the culture revealed that transgenes for BMP-2 and BMP-2 + VEGF induced more mRNA expression of alkaline phosphatase, collagen type I, and osteocalcin than VEGF and vehicle treatments; additionally, alizarin red S staining, alkaline phosphatase staining, and alkaline phosphatase activity were significantly higher in the BMP-2 + VEGF transgene than in the other versions. After implantation, ectopic bone was observed at 4 weeks and greatly increased at 8 weeks in all groups. In particular, the combination of BMP-2 and VEGF formed significantly more bone at 4 weeks, and VEGF transfection resulted in more blood vessels relative to the conditions without VEGF. Thus, VEGF might enhance BMP2-induced bone formation through modulation of angiogenesis.

• 社団法人 日本薬理学会の論文
• 2008-09-20

著者

• Ohya K

  Section Of Pharmacology Department Of Hard Tissue Engineering Graduate School Tokyo Medical And Dent

• Ohya Keiichi

  Section Of Pharmacology Department Of Hard Tissue Engineering Division Of Bio-matrix Graduate School

• Ohya K

  Tokyo Medical And Dental Univ. Tokyo Jpn

• Ohya Keiichi

  Pharmacology Department Of Hard Tissue Engineering Graduate School Tokyo Medical And Dental Universi

• Ohya Keiichi

  Pharmacology Department Of Hard Tissue Engineering Graduate School Tokyo Medical And Dental Universi

• Ohya K

  Tokyo Medical And Dental Univ.

• Shimokawa Hitoyata

  Section of Pharmacology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and D

• Shimokawa Hitoyata

  Pharmacology Department Of Hard Tissue Engineering Division Of Bio-matrix Graduate School Tokyo Medi

• Shimokawa Hitoyata

  Section Of Pharmacology Department Of Hard Tissue Engineering Tokyo Medical And Dental University

• Shimokawa Hitoyata

  Section Of Pharmacology Department Of Hard Tissue Engineering Graduate School Tokyo Medical And Dent

• Shimokawa Hitoyata

  Department Of Biochemistry Tokyo Medical And Dental University School Of Dentistry

• Ohya Keiichi

  Department Of Hard Tissue Engineering Section Of Pharmacology Tokyo Medical And Dental University Gr

• Kuroda Shinji

  Section Of Oral Implantology And Regenerative Dental Medicine Department Of Masticatory Function Reh

• Kasugai S

  Section Of Oral Implantology And Regenerative Dental Medicine Department Of Masticatory Function Reh

• SAMEE Mayurach

  Section of Oral Implantology and Regenerative Dental Medicine, Department of Masticatory Function Re

• KASUGAI Shohei

  Section of Oral Implantology and Regenerative Dental Medicine, Department of Masticatory Function Re

• KONDO Hisatomo

  Section of Oral Implantology and Regenerative Dental Medicine, Department of Masticatory Function Re

• Samee Mayurach

  Section Of Oral Implantology And Regenerative Dental Medicine Department Of Masticatory Function Reh

• Kondo Hisatomo

  Section Of Oral Implantology And Regenerative Dental Medicine Department Of Masticatory Function Reh

• Ohya Keiichi

  Pharmacology Department Of Hard Tissue Engineering Division Of Bio-matrix Graduate School Tokyo Medi

• Ohya Keiichi

  Section Of Pharmacology Dep. Of Hard Tissue Engineering Div. Of Bio-matrix Graduate School Tokyo Med

• Kasugai S

  Section Of Oral Implantology And Regenerative Dental Medicine Department Of Masticatory Function Reh

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