Inhibitory Effect of the Phosphoinositide 3-Kinase Inhibitor LY294002 on Muscarinic Acetylcholine Receptor-Induced Calcium Entry in PC12h
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概要
- 論文の詳細を見る
Phosphoinositide-3 kinase (PI3K) and phospholipase C (PLC) utilize the same phosphoinositides as substrates to produce different signaling molecules. These enzymes are activated by a similar set of cell signaling mechanisms, i.e., tyrosine kinases and G proteins, and affect common cell functions, including proliferation, motility, and intracellular trafficking. Despite these similarities, the interplay between these enzymes is not well understood. To address this issue, the effects of the PI3K inhibitor LY294002 on carbachol-induced calcium increase in PC12h cells were examined. As carbachol stimulates both Gq- and Gi-coupled muscarinic acetylcholine receptors (mAChRs), PI3K and PLC are activated simultaneously in this protocol. LY294002 was found to reduce the carbachol-induced calcium increase, and the reduction was attributed to suppression of calcium entry. As LY294002 did not affect either carbachol-induced calcium release or calcium entry induced by calcium store depletion, this agent was found to suppress calcium entry directly activated by mAChRs. Although PI3K was supposed to compete for substrates with PLC, the PI3K inhibitor did not enhance PLC-dependent cellular responses. As LY294002 was still effective by treating cells after carbachol stimulation, it is likely that this agent blocks the calcium entry channels directly.
- 社団法人 日本薬理学会の論文
- 2007-11-20
著者
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MORITA MITSUHIRO
Department of Orthopaedic Surgery, Fujita Health University
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Kudo Yoshihisa
Laboratory Of Cellular Neurobiology School Of Life Science Tokyo University Of Pharmacy And Life Sci
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MORITA Mitsuhiro
Laboratory of Cellular Neurobiology, School of Life Science, Tokyo University of Pharmacy and Life S
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Morita M
Department Of Orthopaedic Surgery Fujita Health University
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Nakane Akira
Laboratory Of Cellular Neurobiology School Of Life Science Tokyo University Of Pharmacy And Life Sci
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YOSHIZAKI Kotaro
Laboratory of Cellular Neurobiology, School of Life Science, Tokyo University of Pharmacy and Life S
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Kudo Y
Laboratory Of Cellular Neurobiology School Of Life Science Tokyo University Of Pharmacy And Life Sci
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