Effects of Bifemelane on the Calcium Level and ATP Release of the Human Origin Astrocyte Clonal Cell
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概要
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The effect of bifemelane hydrochloride (bifemelane) was examined on human origin astrocyte clonal cells (Kings-1). Bifemelane (125 – 1000 μM) induced a dose-dependent increase in the intracellular calcium concentration ([Ca2+]i). In the highest concentration (1000 μM), the drug caused the second large increase in [Ca2+]i during the washing. The increase that occurred during the administration partially remained in the Ca2+-free medium and was blocked by 2-aminoethoxydiphenyl borate (2-APB), an IP3-receptor blocker, indicating that the source of Ca2+ for the increase could be ascribed to the intracellular store. The increase in [Ca2+]i was not observed during washing with Ca2+-free medium, but was observed when the washing was performed with Ca2+-containing medium. Bifemelane caused a dose-dependent ATP release, but histamine and carbachol, which induced a large increase in [Ca2+]i, had no effects on the ATP release. The effects on the [Ca2+]i were blocked by pretreatment with pyridoxal phosphate-6-azophenyl-2,4 disulfonic acid, a P2-receptor antagonist. Although the mechanisms of ATP release induced by the drug have not been elucidated yet, the present results demonstrate that the increase in [Ca2+]i induced by bifemelane is not due to its direct effect on the cells, but is dependent upon the ATP released from the cells.
- 社団法人 日本薬理学会の論文
- 2006-09-20
著者
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MORITA MITSUHIRO
Department of Orthopaedic Surgery, Fujita Health University
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Yoshida Yoshitoku
School Of Life Science Tokyo University Of Pharmacy And Life Science
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KUMAGAI Haruno
School of Life Science, Tokyo University of Pharmacy and Life Science
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OHKUBO Yoshiumi
School of Life Science, Tokyo University of Pharmacy and Life Science
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TSUCHIYA Remi
School of Life Science, Tokyo University of Pharmacy and Life Science
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MORITA Mitsuhiro
School of Life Science, Tokyo University of Pharmacy and Life Science
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MIYAKAWA Hiroyoshi
School of Life Science, Tokyo University of Pharmacy and Life Science
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KUDO Yoshihisa
School of Life Science, Tokyo University of Pharmacy and Life Science
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Tsuchiya Remi
School Of Life Science Tokyo University Of Pharmacy And Life Science
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Morita M
Department Of Orthopaedic Surgery Fujita Health University
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Kumagai Haruno
School Of Life Science Tokyo University Of Pharmacy And Life Science
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Kudo Y
Laboratory Of Cellular Neurobiology School Of Life Science Tokyo University Of Pharmacy And Life Sci
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Miyakawa H
School Of Life Science Tokyo University Of Pharmacy And Life Science
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Kudo Yoshihisa
School Of Life Science Tokyo University Of Pharmacy And Life Science
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Ohkubo Yoshiumi
School Of Life Science Tokyo University Of Pharmacy And Life Science
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