Reduced immunoreactivity of urinary albumin in patients with cardiovascular diseases : Analysis of immunochemically nonreactive albumin
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概要
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We analyzed 55 spot urine samples from patients with cardiovascular diseases. Urinary albumin concentrations were measured with size exclusion high performance liquid chromatography (HPLC), turbidimetric immunoassay (TIA) using anti human serum albumin polyclonal antibody, and enzyme-linked immunosorbent assay (ELISA) using anti human serum albumin monoclonal antibody. Fractionated urine samples from the HPLC were also analyzed with the immunoprecipitation reactions using as same monoclonal antibody as ELISA. As a result, the urinary albumin concentration analyzed by the HPLC was systematically higher than that of immunoassays, however, ‘albumin peak’ from the HPLC contained other urinary proteins. Result of immunoprecipitation reaction showed the presence of monomer albumin that could not react with the monoclonal antibody. These results suggest that not only contamination of other proteins, the albumin fraction from the HPLC included albumin with reduced its reactivity to the specific monoclonal antibody.
- 2008-09-01
著者
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Isobe Mitsuaki
Cardiovascular Medicine, Tokyo Medical and Dental University
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NAKAYAMA Aki
Life Science and Bio-informatics, Division of Biomedical Laboratory Sciences, Graduate School of Hea
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NISHIMAKI Jyunichi
Life Science and Bio-informatics, Division of Biomedical Laboratory Sciences, Graduate School of Hea
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KAWARA Tokuhiro
Life Science and Bio-informatics, Division of Biomedical Laboratory Sciences, Graduate School of Hea
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KASAMA Takeshi
Moleculo-genetic Sciences, Division of Biomedical Laboratory Sciences, Graduate School of Health Sci
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BABA Toshiaki
Research & Development Laboratory, NIPRO CORPORATION
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YOSHIDA Hiroshi
Research & Development Laboratory, NIPRO CORPORATION
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SHIBA Kiyoko
Faculty of Health Science Technology, Bunkyo Gakuin University
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SATO Kenji
Life Science and Bio-informatics, Division of Biomedical Laboratory Sciences, Graduate School of Hea
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Kawara Tokuhiro
Tokyo Medical And Dental Univ.
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Kawara Tokuhiro
Department Of Biofunctional Informatics Graduate School Of Health Care Sciences Tokyo Medical And De
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Kawara Tokuhiro
Life Science And Bio-informatics Division Of Biomedical Laboratory Sciences Graduate School Of Healt
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Kozu Taeko
武蔵野赤十字病院 循環器科
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Nakayama Aki
Life Science And Bio-informatics Division Of Biomedical Laboratory Sciences Graduate School Of Healt
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Kasama Takeshi
Moleculo-genetic Sciences Division Of Biomedical Laboratory Sciences Graduate School Of Health Scien
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Baba Toshiaki
Research & Development Laboratory Nipro Corporation
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Shiba Kiyoko
Faculty Of Health Science Technology Bunkyo Gakuin University
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Nishimaki Jyunichi
Life Science And Bio-informatics Division Of Biomedical Laboratory Sciences Graduate School Of Healt
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Isobe Mitsuaki
Cardiovascular Medicine Division Of Systemic Organ Regulation Tokyo Medical And Dental University Gr
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Sato Kenji
Life Science And Bio-informatics Division Of Biomedical Laboratory Sciences Graduate School Of Healt
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Sato Kenji
Life Science And Bio-informatics Division Of Biomedical Laboratory Sciences Graduate School Of Healt
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Yoshida Hiroshi
Research & Development Laboratory Nipro Corporation
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Shiba Kiyoko
Faculty Of Health Care Science Technology Bunkyo Gakuin University
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Sato Kenji
Life Sci. And Bio-informatics Div. Of Biomedical Lab. Sciences Graduate School Of Health Sciences Tokyo Medical ...
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