Expression patterns of Arabidopsis ERF VIII-b subgroup genes during in vitro shoot regeneration and effects of their overexpression on shoot regeneration efficiency
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概要
- 論文の詳細を見る
The Arabidopsis ESR1, which encodes a member of the ERF family, is thought to be a key gene for commitment to shoot differentiation in tissue culture. The Arabidopsis genome has more than a hundred genes encoding the ERF family and ESR1 belongs to the ERF VIII-b subgroup. We examined ESR1 and 5 genes structurally similar to ESR1 (ESR2, ESL1, ESL2, ESL3 and LEP) on their expression patterns during in vitro shoot regeneration. All these genes transcript levels increased 1–2 days after shoot induction by incubation on shoot-inducing medium and then decayed by day 7. These genes overexpression demonstrated that ESR1 and ESR2 clearly enhanced shoot regeneration when overexpressed, but other genes did not. These results suggest that all 6 examined genes may be involved in early events of shoot regeneration, although only ESR1 and ESR2 enhanced shoot regeneration by their overexpression.
- 2007-12-01
著者
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MATSUO Naoki
Plant Biology Research Center, Chubu University
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MASE Hiromi
Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University
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BANNO Hiroharu
Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University
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Matsuo Naoki
Department Of Pediatrics Gifu Prefectral Gifu Hospital
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Mase Hiromi
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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Banno Hiroharu
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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HASHIBA Miki
Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University
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Hashiba Miki
Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University
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MATSUO Naoki
Department of Civil Engineering, Chubu University
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