Analysis of functional domains and binding sequences of Arabidopsis transcription factor ESR1

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概要

• 論文の詳細を見る

• The Arabidopsis ESR1 is thought to be a key gene for commitment to in vitro shoot regeneration in tissue culture. The ESR1 gene encodes a putative transcription factor. We examined the DNA-binding potential for specific sequences. Recombinant ESR1 protein specifically bound to the GCC box, known as the ethylene-responsive element. In addition, ESR1 was localized to the nucleus when expressed in onion epidermal cells. Deletion experiments revealed that the DNA-binding domain or the C-terminal region of ESR1 is essential for ESR1 overexpression to enhance shoot regeneration. The C-terminal region may be involved in transcriptional regulation by ESR1. These results suggest that ESR1 functions as a transcription factor and that its transcriptional regulation is important for the initiation of shoot regeneration.

• 日本植物細胞分子生物学会の論文
• 2006-06-01

著者

• MASE Hiromi

  Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University

• BANNO Hiroharu

  Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University

• Mase Hiromi

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

• Banno Hiroharu

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

• Maekawa Koji

  Department of Thoracic and Cardiovascular Surgery, Sapporo Medical College

• Maekawa Koji

  Department Of Environmental Biology College Of Bioscience And Biotechnology Chubu University

関連論文

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• Expression patterns of Arabidopsis ERF VIII-b subgroup genes during in vitro shoot regeneration and effects of their overexpression on shoot regeneration efficiency
• Analysis of functional domains and binding sequences of Arabidopsis transcription factor ESR1

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