腱板修復に関する実験的研究 : 滑膜組織の影響について

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The purpose of this study was to clarify the effects of synovial tissue and growth factor on the healing process of the rotator cuff in a rat model. A 3mm-diameter full thickness defect at the supraspinatous tendon was created in 48 rats. They were classified into the following 2 groups : F group with only defect, S group with defect filled with synovial tissue of the knee. The shoulder were removed at 1,2,4 and 8weeks after surgery. The specimens were investigated using H-E staining, immunostaining with anti-bodies against proliferating cell nuclear antigen (PCNA), TGF β-1, bFGF and in situ hybridization using the cRNA probe for the procollagen types I and III. Each sample was evaluated by microscopic examination using a semiquantitative scale of histologic tendon mature grading, the percentage of positively stained cells. The data were analyzed by Mann-Whitneys U- test. On macroscopic evaluation, group S had a closure of defects at 4 weeks but group F had persistent defects of the articular side at 8 weeks. The histologic tendon mature grading and the percentage of positively stained cells of the PCNA were higher in group S than in group F throughout all periods. At 1 and 2 weeks, the histologic tendon mature score for group S was significantly higher than that for group F. Signals of procollagen types I and III for group S were detected in tissues whole around the defect area, whereas those for group F were localized in tissue adjusted to the bursal side and articular side. Expressions of bFGF were detected in tissues whole around the defect areas in both groups, whereas those of TGF β-1 were detected marked higher in group S than in group F. Our findings indicate that the synovium plays an important role in enhancing the tendon healing capacity and expressions of TGF β-1 may affect the synovial effect of the healing process for the rotator cuff.
日本肩関節学会の論文
2002-01-01

腱板修復に関する実験的研究 : 滑膜組織の影響について

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