胎生期ウシ気管軟骨の基質小胞に関する研究 : 新しいタイプの基質小胞について

概要

論文の詳細を見る
Matrix vesicles are extracellular organelles that initiate mineralization in the extracellular matrix of various calcifying tissues. It is generally accepted that matrix vesicles arise by budding and pintching off from the cell membrane and therefore matrix vesicles contain a high level of alkaline phosphatase (ALP). In recent years, lactate dehydrogenase (LDH) isoenzymes were found to be located in matrix vesicles of epiphyseal growth plate of young rabbit leg bone and metalloprotease in matrix vesicles of chicken epiphyseal cartilage. On the other hand, calcification of the trachea cartilage has been reported although it was once accepted to remain as a permanent hyaline cartilage without calcification. In the present study, properties of matrix vesicles in the trachea cartilage of fetal bovine were compared with those in the epiphyseal cartilage of tibia in 2-month-old fetal bovine. 1. Only the matrx vesicles with ALP, LDH, and metalloprotease were present in the epiphyseal cartilage of tibia. In contrast, there were not only the matrix vesicles but also matrix vesicle-like vesicles with a high density in the trachea cartilage ; the vesicle contained ALP and LDH but did not metalloprotease. 2. Aldolase, aspartate : 2-oxoglutarate aminotransferase, alanine : 2-oxoglutarate aminotransferase, cytosolic enzymes except LDH, were not detected in these vesicles. These results suggested the presence of mechanism for the specific uptake of cytosolic LDH. 3. The electrophoretic LDH isoenzyme patterns differed between the matrix vesicles and the matrix vesicle-like vesicles in the tracheal cartilage. 4. There were also not only the matrix vesicles but also matrix vesicle-like vesicles with a high density in the 5-month-old fetal trachea cartilages.
九州歯科学会の論文
1997-10-25

胎生期ウシ気管軟骨の基質小胞に関する研究 : 新しいタイプの基質小胞について

スポンサーリンク

概要

著者

関連論文

スポンサーリンク