エナメル芽細胞の分化を観察するための培養法の確立

概要

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The culture system is a very useful method for elucidating differentiation mechanisms of ameloblast which is associated with induction of odontoblast and/or production of enamel matrix. The aim of this study is establishment a primary cell culture system using isolated cervical loop and dental mesenchymal cells in growing end of rat incisors, which make it possible to observe the process of ameloblast cell lineage. To monitor growth and differentiation of epithelial cells, antibodies against cytokeratin 14 and ameloblastin were used as markers of dental epithelial cells and ameloblasts respectively. In this culture, the number of epithelial cells gradually decreased and became 50% at 10 days. However, all of their remaining cells were ameloblastin expressing cells. When cells were cultured in the presence of skin fibroblasts, they did not express ameloblastin. This culture system is useful for investigation of ameloblast differentiation mechanism in the epithelial-mesenchymal interactions.
九州歯科学会の論文
2002-02-25