Flow Cytometry for Rapid Determination of Number of Microbial Cells Grown on Fish
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概要
- 論文の詳細を見る
Application of the flow cytometry (FCM) technique for rapid determination of the total number of microbial cells grown on yellow-fin horse mackerel was described. An ultrasonic treatment was used to remove the cells on seafood samples of yellow-fin horse, Japanese common squid, short-neck, and common octopus. Almost of cells were removed from the samples in 9 min by the treatments. Since seafood products might contain various non-microbial debris particles in addition to microbial cells, propidium iodide was used to discriminate the two groups. A fluorescence histogram distinguished the cells from the particles. The FCM method allowed determination of the number of microbial cells adhering to fish during the preservation period. Cell numbers determined by FCM well paralleled that measured by the raditional colony counting method in the range of 105–109 cells/g. One FCM assay could be completed within 60 s and the total assay time including preparation of the microbial cells was within 30 min.
- 社団法人 日本食品科学工学会の論文
- 2002-11-01
著者
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REN Huifeng
Department of Marine Science, Tokyo University of Marine Science and Technology (TUMST)
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HAYASHI Tetsuhito
Department of Marine Science, Tokyo University of Marine Science and Technology (TUMST)
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Endo Hideaki
Department Of Cardiology Tohoku University
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Ren Huifeng
Department Of Marine Science Tokyo University Of Marine Science And Technology (tumst)
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Ren Huifeng
Department Of Ocean Sciences Tokyo University Of Marine Science And Technology
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Endo H
Department Of Marine Science Tokyo University Of Marine Science And Technology (tumst)
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Endo Hideaki
Department Of Ocean Sciences Tokyo University Of Marine Science And Technology
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Ren H
Department Of Marine Science Tokyo University Of Marine Science And Technology (tumst)
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Ren Huifeng
Department Of Food Science & Technology Tokyo University Of Fisheries
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NAKAMURA Junichi
Departments of Neurosurgery and Otorhinolaryngology, Nakamura Memorial Hospital
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Hayashi Tetsuhito
Department Of Marine Science Tokyo University Of Marine Science And Technology (tumst)
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Hayashi Tetsuhito
Department Of Food Science & Technology Tokyo University Of Fisheries
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Nakamura Junichi
Department Of Materials Science The University Of Shiga Prefecture
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Nakamura Junichi
Department Of Electrical Engineering Hiroshima University
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Nakamura Junichi
Department Of Food Science And Technology Tokyo University Of Fisheries
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Endo Hideaki
Faculty Of Marine Science Tokyo University Of Marine Science And Technology
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Hayashi Tetsuhito
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
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Nakamura Junichi
Department of Chemical Engineering, Graduate School of Science and Engineering, Tokyo Institute of Technology
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