Electrokinetic Delivery of Biomolecules into Living Cells for Analysis of Cellular Regulation

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概要

• 論文の詳細を見る

• Current biomolecule-delivery systems, which function via pressure-driven flow, possess several significant limitations. We herein present a novel delivery method for biomolecules based on electrokinetic flow. The electrokinetic flow method uses capillaries to more easily deliver solutions than the pressure-driven flow method, which enables controllable injection of biomolecules into living cells. We characterized the electrokinetic flow of molecules to determine the parameters required for reproducible injections. Then, we successfully demonstrated the electrokinetic injection of a fluorescent DNA and dye solution into a living HeLa cell through a glass needle (1 μm in diameter). The monitoring of changes in ion current during the delivery process also allowed for the precise detection of penetration through the cell membrane with the tip of the needle.

• The Japan Society of Applied Physicsの論文
• 2013-04-25

著者

• Shibata Takayuki

  Department Of Energy Engineering & Science Faculty Of Engineering Nagoya University

• Kawashima Takahiro

  Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University

• Nagai Moeto

  Department of Mechanical Engineering, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan

• Torimoto Tatsuro

  Department of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan

• Miyamoto Tokuma

  Department of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan

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