食品中の黄色ブドウ球菌毒素エンテロトキシンの検出に関する研究--エンテロトキシン精製における力価の変動

概要

論文の詳細を見る
Testing for enterotoxin S. aureus in food with low levels of the poison using the reversed passive latex agglutination (RPLA) method does not produce detectable results that correlate with symptoms. The purpose of the current investigation is to discuss the concentration of samples and show how this provides for detectable levels of measurement. Acid precipitation was used to provide concentrated purified samples of entrotoxin and isoelectric point of protein dialysis methods applied to provide results. The recovered enterotoxin value was then measured with RPLA and the margin of error was shown to be less than double the value fo that obtatned for the control sample and its recovery rate was 50-80%. The symptom level for S. aureus poisoning is at 0.1ng/ml but the detection limit of the RPLA test kit was about 1ng/ml so experiment seems to indicate that concentration levels of 10 times are required to measure enterotoxins in food.
関東学院大学人間環境学部人間環境学会の論文