Orientation of a Dip-Coated Bacteriorhodopsin Thin Film Studied by Second Harmonic Generation Interferometry
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概要
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We investigated the absolute orientation of bacteriorhodopsin (bR) thin film prepared by the dip-coating technique by means of second harmonic generation (SHG) interferometry. bRs in the bR thin films prepared in this technique tend to have a preference to orient with the cytoplasmic side away from the glass substrate and extracellular side toward the glass substrate. To assist in the analysis, the hyperpolarizability of covalently bound retinal chromophore was also evaluated by a computational calculation.
- 2013-05-25
著者
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Otomo Akira
National Inst. Information And Communications Technol. Kobe Jpn
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Yamada Toshiki
National Institute Of Information And Communications Technology
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Kikuchi Hiroshi
Nhk Science & Technical Research Laboratories
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Tanaka Shukichi
National Institute of Information and Communication Technology, Kobe Advanced Research Center, Kobe 651-2492, Japan
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Terui Toshifumi
National Institute of Information and Communication Technology, Kobe Advanced Research Center, Kobe 651-2492, Japan
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Haruyama Yoshihiro
National Institute of Information and Communications Technology, Kobe 651-2492, Japan
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Kasai Katsuyuki
National Institute of Information and Communications Technology, Kobe 651-2492, Japan
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Kaji Takahiro
National Institute of Information and Communications Technology, Kobe 651-2492, Japan
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