Ultrasound-Mediated Gene Transfection In vitro: Enhanced Efficiency by Complexation of Plasmid DNA
スポンサーリンク
概要
- 論文の詳細を見る
Ultrasound-mediated gene transfection in the presence of microbubbles is a recently developed promising nonviral gene delivery method. The main obstacle towards its clinical application is its low transfection efficiency. In this work, we investigate the effect of the complexation of plasmid DNA (pDNA) into polyplex micelles on the transfection efficiency. Complexation changes the structure of pDNA and results in the condensation in size and enhanced stability. Both naked and complexed pDNAs were transfected into cultured cells using ultrasound in the presence of microbubbles. The transfection rate using complexed pDNA is considerably enhanced (from {\sim}0.92 to {\sim}1.67%, by {\sim}82%) compared with the rate using naked pDNA. Our method provides an alternative for the improvement of the transfection efficiency of the ultrasound-mediated method.
- 2012-07-25
著者
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Okamoto Akio
Department Of Human And Mechanical Systems Engineering Faculty Of Engineering Kanazawa University
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Osada Kensuke
Department Of Polymer Chemistry Faculty Of Engineering Tokyo Institute Of Technology
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Azuma Takashi
Department Of Cardiovascular Surgery Toyama Prefectural Central Hospital
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Kataoka Kazunori
Department Of Chemistry Graduate School Of Science Osaka University
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Sasaki Akira
Department Of Applied Physics Tokyo Institute Of Technology
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Matsumoto Yoichiro
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Takagi Shu
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Zhang Yiwei
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Tachibana Rie
Department of Mechanical Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Yoshinaka Kiyoshi
The National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8564, Japan
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Osada Kensuke
Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Azuma Takashi
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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Okamoto Akio
Department of Mechanical Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan
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