High-Frequency Single-Cell Isolation of Bacteria Using Microenclosure Array with Multipillar Structure

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概要

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• We proposed and demonstrated the single-cell isolation of bacteria using a microenclosure array with a structure composed of many micropillars. We fabricated the microenclosure array on a semiconductor wafer by electron beam lithography and the dry etching technique. The capturing frequency for single cells of Escherichia coli was approximately 50% using a 4-μm-width microenclosure array. We think that the proposed novel technique is very simple and useful for the single-cell isolation of many kinds of bacteria using a microenclosure array of optimum size.

• 2010-12-25

著者

• Takada Ayako

  Bio-Technical Center, Technical Department, Tokyo Institute of Technology, B-41, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan

• Matsutani Akihiro

  Center for Semiconductor and MEMS Processes, Technical Department, Tokyo Institute of Technology, R2-3, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan

• Matsutani Akihiro

  Center for Semiconductor and MEMS Processes, Technical Department, Tokyo Institute of Technology, R2-21, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan

関連論文

• High-Frequency Single-Cell Isolation of Bacteria Using Microenclosure Array with Multipillar Structure
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