In situ Surface Infrared Study of DNA Hybridization on Au Island Films Evaporated on Silicon Surfaces

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概要

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• Here we propose a label-free detection of DNA hybridization on Au nanoisland films deposited on Si surfaces by infrared absorption spectroscopy in the geometry of multiple internal reflection (MIR-IRAS). Au nanoisland films were prepared on Si surfaces by evaporation. DNA immobilization via Au-thiol chemistry and subsequent DNA hybridization was monitored with MIR-IRAS. Interaction with complementary DNA produced an IRAS peak at ${\sim}1686$ cm-1 where the C=O stretching vibration modes of nucleobases appear. On the other hand, addition of non-complementary DNA induced no significant spectral changes in the same region. Sensitive detection of 40 nM DNA was achieved owing to the MIR geometry combined with Au nanoisland films deposited on Si which provides low-background absorption around the C=O stretching vibration region. Comparison between experimental results and theoretical prediction, obtained using the ab initio calculation, was also examined.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2009-04-25

著者

• KIMURA Yasuo

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Toho

• NIWANO Michio

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Toho

• Hirano-iwata Ayumi

  Graduate School Of Biomedical Engineering Tohoku University

• Hirano-Iwata Ayumi

  Graduate School of Biomedical Engineering, Tohoku University, 6-6 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan

• Miyamoto Ko-ichiro

  Department of Electronic Engineering, Graduate School of Engineering, Tohoku University, 6-6-05 Aza-Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan

• Tanaka Kohki

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan

• Kimura Yasuo

  Laboratory for Nanoelectronics and Spinelectronics, Research Institute of Electrical Communication, Tohoku University

• Niwano Michio

  Laboratory for Nanoelectronics and Spinelectronics, Research Institute of Electrical Communication, Tohoku University

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