Area Expansivity Moduli of Regenerating Plant Protoplast Cell Walls Exposed to Shear Flows

概要

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To control the elasticity of the plant cell wall, protoplasts isolated from cultured Catharanthus roseus cells were regenerated in shear flows of 115 s-1 (high shear) and 19.2 s-1 (low shear, as a control). The surface area expansivity modulus and the surface breaking strength of these regenerating protoplasts were measured by a micropipette aspiration technique. Cell wall synthesis was also measured using a cell wall-specific fluorescent dye. High shear exposure for 3 h doubled both the surface area modulus and breaking strength observed under low shear, significantly decreased cell wall synthesis, and roughly quadrupled the moduli of the cell wall. Based on the cell wall synthesis data, we estimated the three-dimensional modulus of the cell wall to be $4.1\pm 1.2$ GPa for the high shear, and $0.35\pm 0.2$ GPa for the low shear condition, using the surface area expansivity modulus divided by the cell wall thickness, which is identical with the Young's modulus divided by $2(1-\sigma)$, where $\sigma$ is Poisson's ratio. We concluded that high shear exposure considerably strengthens the newly synthesized cell wall.
Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
2005-05-15