AP-1-mediated expression of brain-specific class IVa β-tubulin in P19 embryonal carcinoma cells

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概要

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• Expression of brain-specific phenotypes increased in all trans retinoic acid (ATRA)-induced neural differentiation of mouse P19 embryonal carcinoma cells. Among these phenotypes, expression of class IVa β-tubulin isotype (TUBB4a) was particularly enhanced in neural differentiation. Transient transfection assays employing a reporter construct found that ATRA-mediated regulatory region of the TUBB4a gene lay in the region from −83 nt to +137 nt relative to the +1 transcription start site. Site-directed mutagenesis in the AP-1 binding site at −29/−17 suggested that the AP-1 binding site was a critical region for ATRA-mediated TUBB4a expression. Chromatin immunoprecipitation experiments suggested participation of JunD and activating transcription factor-2 (ATF2) in TUBB4a expression. Additionally, exogenous induction of the dominant-negative (dn) type of JunD canceled ATRA-induced upregulation of TUBB4a, and the dn type of ATF2 suppressed even the basal activity. Further immunoblot study revealed an ATRA-mediated increase in JunD protein, while a significant amount of ATF2 protein was constantly produced. These results suggest that differentiation-mediated activation of JunD results in enhanced TUBB4a expression.

• 公益社団法人 日本獣医学会の論文

著者

• Arai Katsuhiko

  Department of Chemistry, Faculty of Science, Toho University

• MARUYAMA Yuka

  Department of Tissue Physiology, Tokyo University of Agriculture and Technology

• ARAHARA Kazuhiko

  Department of Tissue Physiology, Tokyo University of Agriculture and Technology

• KINOSHITA Emi

  Department of Tissue Physiology, Tokyo University of Agriculture and Technology

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