Fine Structural Analysis of the Unique 5' Region of the Human COL1A2 Gene Containing Two Regions of Dinucleotide Repeats Adjacent to the Transcriptional Start Site

概要

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To investigate the mechanism of corrdinated transcription of the human type I collagen α1(COL1A1)and α2(COL1A2)genes, we determined the sequence of the presumptive transcriptional regulatory region, from 3.6 kilo base pairs(kbp)upstream from the transcriptional start site to the 3' end of the 3rd intron, of the human COL1A2 gene, and compared it with that of the corresponding regions of the human COL1A1 and the mouse colla2 gene. A distinctive feature of the human COL1A2 gene was the presence of two regions of dinucleotide repeats that were not found in the human COL1A1 gene. Dot matrix analysis indicated that there were highly homologous regions between the nucleotide sequences proximal to the transcriptional start site of the human COL1A2 and mouse colla2 genes. On the other hand, there were apparently no highly homologous nucleotide sequences in the 5' flanking region or in the 1st intron between the COL1A2 and COL1A1 genes. These results suggest that the cooperative expression of these genes is regulated not due to a simple homology of the cis-regulatory elements but is controlled by a complex combination of several cis-regulatory elements and trans-acting factors that interact with these elements.
日本結合組織学会の論文
1998-03-25