上咽頭癌におけるEBER-1核内小RNAの検索

概要

論文の詳細を見る
Nasopharyngeal carcinoma (NPC) is closely related with Epstein-Barr virus (EBV). In latent EBV-infection of cells, EBV-encoded small RNAs (EBERs) are abundantly expressed, approximately 10<SUB>7</SUB> copies per cell. We examined the detection of EBER-1 which is one of two EBERs using the in situ hybridization method in 5 cases of formalin-fixed, paraffin-embedded NPC tissues. Specimens consisted of 5 primary nasopharyngeal carcinoma tissues (NP),3 metastatic cervical lymph nodes (MCL) and 1 non-metastatic cervical lymph node. EBER-1 expression was strongly demonstrated in 4 of 5 NP and 2 of 3 MCL. Positive signals were only shown within the nuclei of tumor cells. Anti-EBV antibody titer was investigated in 3 cases,2 EBER-1 positive cases and 1negative case. The titer, especially against VCA-IgA and EA-IgA, was high in the 2 EBER-1positive cases and low in the negative case. Thus, in situ hybridization with an EBER-1 probe is a sensitive and specific method of detecting EBV in formalin-fixed, paraffin-embedded NPC tissues.
耳鼻咽喉科臨床学会の論文