内耳微小循環障害によるラット難聴モデル

概要

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We produced a new model of hearing disturbance in rats due to photochemically induced thrombosis in inner ear microcirculation. Under sodium pentobarbital anesthesia, green light (540 nm) was irradiated on the lateral bony wall, which was exposed by a ventrolateral approch, while rose bengal (RB,20 mg/kg, i. v. ) was injected. The disintegration of hair cells and stria vascularis was observed 24 hours after the completion of the illumination with RB in light microscopy and platelets and fibrin nets, which entrapped the red blood cells, filled in the arteriole of the inner ear in scanning electron microscopy. The compound action potential of the cochlear nerve (AP) was measured every minute after the injection of RB. The AP disappeared about 4 minutes after the administration of RB. The time required to completely suppress the AP was significantly prolonged in animals treated with aspirin (30 mg/kg), which was injected 5 minutes before the administration of RB, as compared with controls. In conclusion, our method causes a photochemically induced occlusion in inner ear's microcirculation and is potentially usefull for evaluating the various effects of drugs on inner ear's microcirculation disorders.
耳鼻咽喉科臨床学会の論文