Cytotoxicity of Photosensitizing Crystalline C60 Particles Formed by Ink-jet Method
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概要
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The crystalline C60 particles were synthesized into PDMS-based microwells on microarray chips using an ink-jet spotting system, and the effect of the particles on cytotoxicity was investigated using HeLa cells (human cervical carcinoma cell). It was found from SEM observations that a number of C60 particles were formed along the edges of 300μm microwells, which could have enough area to encapsulate several tens of cells in the center. The tests with HeLa cells revealed that the C60 particles irradiated green laser caused cytotoxicity, and the rate of cell death was controlled by the irradiation time. It was suggested that the cytotoxicity was induced by reactive oxygen species (ROS) generated by the C60 particles, and an effect of the ROS on the cells covered a large part of the microwell.
- The Imaging Society of Japanの論文
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