A Preliminary Study of the Effect of Static Magnetic Field Acting on Rat Bone Marrow Mesenchymal Stem Cells during Osteogenic Differentiation In Vitro

概要

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Bone marrow mesenchymal stem cells (BMMSCs) could be induced to osteoblasts, which were considered as the seed cells source for organizational engineering. Moreover, it has been demonstrated that Static Magnetic Field (SMF) can stimulate osteoblasts differentiation, but SMF stimulation acting on BMMSCs remained unknown results. The aim of this study is to investigate the effect of SMF acting on rat BMMSCs during osteogenic differentiation in vitro. The experimental BMMSCs were divided into 2 groups, which were experiment group and control group, and both of them were induced by differentiate medium. Experiment group was exposed to SMF at 200 mT 24h/day during osteogenesis. Contrarily, control group was not exposed to SMF during osteogenesis. Results showed that SMF exposure significantly increased the cell proliferation rate, alkaline phosphatase (ALP) activity, as well as the expression of osteogenesis-related proteins. Besides, matrix mineralization was significantly more visible in experiment group than that in control group on the 7th day of the experimentation. In summary, SMF exposure could significantly enhance osteogenic differentiation and mineralization of rat BMMSCs.
硬組織再生生物学会の論文