Preparation of Expanded Poly (tetrafluoroethylene) Films Grafted by Oxygen Plasma Pretreatment and Immobilization of Lipase

概要

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The enzyme activity of lipase, which was covalently immobilized directly or via the spacers (6-AA or HMDA) onto the ePTFE-g-PHEMA films prepared by the combined use of oxygen plasma pretreatment and photografting, was evaluated. The surfaces of ePTFE-g-PHEMA and ungrafted ePTFE films were observed by SEM. Grafted PHEMA chains penetrated into the inside of the membrane with an increase in the grafted amount. Although the optimal pH and temperature of immobilized lipase were the same as those of native lipase, the tolerance to pH persisted in the more wide range. The enzyme activity of immobilized lipase decreased with an increase in the amount of immobilized lipase and immobilization time. About 80% of the initial activity of the immobilized lipase was held even after the immobilized lipase was repeatedly used five times. However, the enzyme activity in prolonged preservation, e.g., more than 30 days, fell significantly.
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