Comparative Study on Amylopectin Molecules of Koshihikari and Reiho (Oryza sativa L. japonica)
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概要
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Amylopectins of Koshihikari and Reiho were partially hydrolyzed with isoamylase immobilized on magnetic supports in order to obtain information on their sectional structures . After stopping the reactions at about 20% hydrolysis, the resulting hydrolysates were separated into three fractions (Fr1, Fr 2 and Fr 3) by gel chromatography with Toyopearl HW 65F. Chain-length distributions of the fractions were investigated by HPAEC-PAD after debranching perfectly with free isoamylase . From a comparison of these HPAEC-PAD data, it was suggested that longish chains are somewhat plentiful in the outer portion of Reiho Ap and that Koshihikari Ap is composed of shortish chains in all portions .
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