Extraction of Trehalose from Saccharomyces cerevisiae by High-Pressure Treatment

概要

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Commercially available yeasts normally contain approximately 10% dry weight of trehalose. For the purpose of obtaining liquid trehalose economically and safely, without using heat or an organic solvent, we used a high-pressure treatment to extract the trehalose, with inactivating trehalase. Our results showed that trehalase is inactivated by the application of a minimum of 700 MPa and that trehalose is not hydrolyzed even at pressures as high as 1500 MPa. Using high-pressure treatment, therefore, we have succeeded in extracting trehalose from yeast at 12% dry weight when 700 MPa is applied for 10 min at 30°C. It has been confirmed that high-pressure treatment is very effective for simultaneously inactivating trehalase and extracting trehalose.
日本応用糖質科学会の論文