Continuous Production of Maltosyl Cyclodextrins Using Immobilized Pullulanase on Novel Ceramic Carrier

概要

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The purpose of the present study was to find a suitable carrier and optimum conditions for the production of maltosyl (α1→6) cyclodextrins (G2-CDs) using immobilized Bacillus acidopullulyticus Pullulanase. A sepiolite treated with acid with a pore size of 600-700Å was the most suitable carrier for the immobilization of pullulanase. The suitable concentration of acetate bufferr and optimum pH for the immobilization was 20 mM and 4, respectively. The temperature did not affect the immobilization within the range of 10-40°C. The optimum amount of adsorbed protein was 30-40 mg/g of carrier . Properties of pullulanase immobilized on the sepiolite carrier under the above condition were investigated. The optimum pH was 4.5 for both immobilized and native enzyme . The optimum temperature of immobilized, enzyme was 60°C, while that of native enzyme was 70°C. The stability of both immobilized and native enzyme was retained within the pH range of 4-6. The G2-CDs synthesizing activities of a column reactor (8.0i.d.×19.9 cm) packed with immobilized pullulanase fell to approximately50% of the initial conversion ratio after 74 days of continuous use at 60°C at a flow rate of SV=0 .072 hr-1 using maltose and cyclodextrin solution [70% (w/w), maltose : cyclodextrins=4 :1; pH5] as the substrate.
日本応用糖質科学会の論文