Ultrastructure and Chemical Analysis of the Cell Wall of Pythium debaryanum

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概要

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• The ultrastructure and component polysaccharides of the cell wall of Pythium debaryanum IFO-5919 were investigated. From results obtained by means of acid, alkali, Schweitzer reagent and β-1, 3-glucanase treatments and electron microscopy, it was concluded that 1) the acid-extracted fraction was a 1, 3-linked branched glucan, 2) the alkali-extracted fraction was a mixture of 1, 3-, 1, 6-, and 1, 3, 6-linked highly branched two glucans, 3) the Schweitzer reagent-extracted fraction was a β-1, 4-linked glucan, 4) the cell wall was constructed from two types of cullulosic microfibrils, as a frame and as a finer network, and amorphous β-1, 3-glucan including β-1, 6-linkage, 5) cellulosic microfibrils were covered by matrix material consisting of a mixture of amorphous β-1, 3-linked and β-1, 6-linked branching glucans.

• 財団法人 学会誌刊行センターの論文

著者

• Miyazaki Toshio

  Department Of Applied Microbiology Nippon Roche Research Center

• YAMADA MICHIHISA

  Department of Physiology, Kurume University School of Medicine

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