Chemical Structure of Arabinogalactan Isolated from Cotyledon of Azuki Bean (Phaseolus angularis)

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Two kinds of acidic arabinogalactan (F2A and F2B) were extracted from the cotyledon of Azuki bean (Phaseolus angularis) with 0.5 M NaCI, and purified by column chromatographies of DEAE-Sephadex A-50 (Cl-form) and Sepharose CL-2B. Both polysaccharides, as constitutional monosaccharides, contained L-arabinose, D-galactose and D-galacturonic acid in a ratio of 13. 15.9 : 1.0 (F2A) and 3.0 : 2.2 : 2.0 (F2B). From the results of methylation and <SUP>13</SUP>C-NMR studies, it could be concluded that F2A and F2B respectively have a backbone consisting of β-(1→3)-linked D-galactose and D-galacturonic acid residues, with α-(1→5)-linked arabinofuranosyl side-chains attached at C6 of D-galactose residue. The molecular weight of F2A and F2B determined by gel permeation chromatography was 50, 000 (F2A) and 94, 000 (F2B), respectively. And on gel permeation chromatographic analysis, elution volume of the polysaccharides was easily influenced by the ion strength of the eluant. This suggests that F2A and F2B could assemble to form a macromolecule through ionic bonds between polysaccharide molecules.
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