Superoxide and Nitric Oxide Production of Kuruma Prawn (Marsupenaeus japonicus) Hemocytes

概要

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The Cypridina luciferin analogue (CLA), a highly sensitive chemiluminescence (CL) probe, was used to detect superoxide (O2) in kuruma prawn hemocytes. The CLA-dependent CL of the shrimp hemocyte after phorbol 12-myristate 13-acetate (PMA) stimulation was inhibited by superoxide dismutase. Nitric oxide (NO) production from the shrimp hemocytes was detected by fluorometric measurement after co-stimulation with lipopolysaccharides (LPS) and recombinant human interferon γ in vitro. The supernatant of a hemocyte culture after stimulation showed an increase in the fluorescence intensity, and this intensity decreased with the addition of NG-monomethyl-L-arginine (L-NMMA). These results show that shrimp hemocytes can produce O2 and NO and these free radicals play an important role in the shrimp defense network.
社団法人日本水産学会の論文