A Biosensor System for the Determination of Cell Number of Enterococcus seriolicida.

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A biosensor system is described for the rapid determination of cell number of Enterococcus seriolicida in a biological sample. The system consisted of E. seriolicida immobilized on a membrane, Clark-type oxygen electrode, and STAN-P culture medium.The STAN-P medium consisted of sodiumazide, a bacterial inhibitor which suppressed the growth of most microorganisms, but not E. seriolicida. The number of E. seriolicida organisms was determined by measuring the oxygen consumption of the cells with an oxygen electrode. The optimum conditions for carrying out the preparation of microorganisms were as follows: incubation temperature, 37°; incubation time, 1 hr, and concentration of sodium aside, 0.3g/l. A linear relationship was obtained between the output current of the sensor andthe number of E. seriolicida cells, which ranged from 1.4×107-7.2×107 cells/ml. The time required fora single assay, including the preparation of microorganisms, was 2 hr.
社団法人日本水産学会の論文