Characterization of Photosynthetic Glycogen and .ALPHA.-Glucosidase from Thermophilic Cyanobacteria, Lyngbya-Phormidium-Plectonema Group B.

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We harvested filamentous cyanobacteria from an alkaline hot spring (45-50°C, pH 8.5-9.0) in Kagoshima Prefecture, and identified it as Lyngbya-Phormidium-Plectonema (LPP) group B by morphological characteristics. From the TCA-soluble fraction of the LPP group B, we isolated glycogen with average chain length of 11 glucose units. The glycogen showed a similar iodine absorption spectrum to that ofrabbit liver glycogen. We also recognized the presence of the TCA-insoluble glycogen at about 48% of total glycogen, most of which was bound non-covalently to protein and showed similar iodine absorption spectrum to that of the TCA-soluble glycogen. We also found thermophilic and thermostable α-glucosidase with optimum pH 6.5 in this cyanobacteria.
社団法人日本水産学会の論文