Levansucraseによるヘテロフルクトオリゴ糖の合成 (アミラ-ゼシンポジウム(1987)<特集>)

概要

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1. Bacillus subtilis levansucrase was isolated from cultured cells grown on a medium containing sucrose at 37°C for 40 hr and purified as an electrophoretically pure state which is free from invertase.2. The enzyme produced levan predominantly toward sucrose alone. But heterofructooligosaccharides were synthesized under the co-existence of mono or oligosaccharide. The maximum efficiency (80%) of transfructosylation from sucrose to acceptor sugars by the enzyme obtained in the case of the acceptor/donor=l or more in molar ratio.3. Oligosaccharide formed by the levansucrase of Bacillus subtilis from sucrose and reducing oligosaccharide was non reducing sugar that C2 of fructosyl residue is linked to Cl of reducing end of acceptor sugars.4. Levansucrase linked to CH-sepharose 4B to produce immobilized enzyme was increased thermal stability 30°C to 40t and used repeatedly to make maltooligosaccharide-F without loss of the activity.5. The susceptibility of oligosaccharide-F to human amylases was tested. Heterooligosaccharides produced by levansucrase can be used as a substrate for screening of new carbohydrolases.
日本応用糖質科学会の論文