Cloning and expression of an alkalophilic Bacillus oligo-1,6-glucosidase gene in Escherichia coli.
スポンサーリンク
概要
- 論文の詳細を見る
The gene coding for an oligo-1, 6-glucosidase of alkalophilic Bacillus sp. F5 was cloned into the EcoRV site of pBR322 and expressed in Escherichia coli HB101.Plasmid pMC1, containing a 9.1Kb EcoRV fragment, was isolated from the α-glucosidase-positive transformant. Genomic hybridization showed that the 9.1Kb EcoRV fragment was derived from the chromosomal DNA of alkalophilic Bacillus F5. Restriction analysis showed that the oligo-1, 6-glucosidase gene was located in the 3.1Kb EcoRV-PvuII fragment. The enzyme productivity of the E. coli HB101 transformant carrying pMC21, which contains a 3.1Kb fragment, was about 13 times higher than that of the DNA donor strain.
- 日本応用糖質科学会の論文
日本応用糖質科学会 | 論文
- 古細菌の産生する新規な糖質関連酵素およびそれらを用いたトレハロースの生産
- PCR法による米 (Oryza sativa L.) の品種判別およびその米加工品への応用
- モチ米のアミロペクチン鎖長分布と米粒の物理特性との関係
- ジャポニカ米とインデカ米の澱粉とプロテインボディの結合脂質について
- 促通拡散型糖輸送担体分子の構造機能相関