高速液体クロマトグラフィ-によるショ糖を結合した水飴中のオリゴ糖の定量について

概要

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Recently we prepared a new syrup from the mixture of starch and sucrose by the action of cyclodextrin glycosyltransf erase. This syrup contained mainly sucrose (GF) and oligosaccharides, such as glucosylsucrose (G2F), maltosylsucrose (G3F) and maltotriosylsucrose (G4F) terminated sucrose at the reducing end. In addition, this syrup contained a small amount of maltooligosaccharides, such as glucose (G), maltose (G2) and maltotriose (G3). In this report, we tried to analyze the sugar composition in this syrup by High Performance Liquid Chromatography. Various sugars, such as fructose (F), G, GF, G2, G2F, G3, G3F were separated and quantitatively determined under the following conditions:, column, μ BONDAPAK/Carbohydrate(4×300 mm) ; solvent system, water/acetonitrile=20/80; flow rate, 2 ml/min or 4 ml/min. Because of less solubility of the oligosaccharides larger than G2F in this solvent system, it was difficult to analyze these oligosaccharides.
日本応用糖質科学会の論文