Gaschromatographic Determination of Trimethylsilyl Derivatives of Maltooligosaccharides
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概要
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Maltosaccharides up to maltohexaose were gaschromatographically determined by use of the severely conditioned short column. Fifty cm of 2% silicone OV-17 column was conditioned at 350°C for 72 hours . This conditioning enabled to elute higher saccharides such as G<SUB>5</SUB> and G<SUB>6</SUB>. The trimethylsilylation procedure essentially as by Brobst was modified into a short time derivatization by micro scale. According to this modified method, maltosaccharides up to G<SUB>6</SUB> are determined less than 2% relative error within 30 min. including the TMS derivative preparation.
- 日本応用糖質科学会の論文
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