Efficient Transfer of Intact Oligonucleotides into the Nucleus of Ligament Scar Fibroblasts by HVJ-Cationic Liposomes Is Correlated with Effective Antisense Gene Inhibition.
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概要
- 論文の詳細を見る
The efficacy of two different cationic liposomes, Lipofectin and hemagglutinating virus of Japan (HVJ)-cationic liposomes, on nuclear uptake of fluorescence-labeled phosphorothioate oligodeoxyribonucleotide (S-ODN) by ligament scar fibroblasts and suppression of decorin mRNA expression when antisense decorin S-ODN was transferred was investigated. There was no significant difference in nuclear uptake of fluorescent ODN between the two methods. However, only HVJ-cationic liposomes had a significant effect on suppression of decorin mRNA expression levels. To address the discrepancy, the molecular integrity of the transferred ODN in the cells was assessed by analysis of fluorescence resonance energy transfer (FRET) within double-fluorescence-labeled S-ODN. More than 70% of the ODN transfected by HVJ-cationic liposomes remained intact within the nucleus at 20h after transfection, while the majority of the ODN transferred by Lipofectin was degraded at this point. These results suggest a strong relationship between the nuclear integrity of transfected antisense ODN and its suppression of target mRNA expression.
- 社団法人 日本生化学会の論文
著者
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SHRIVE Nigel
McCaig Center for Joint Injury and Arthritis Research, University of Calgary
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Hart David
Mccaig Center For Joint Injury And Arthritis Research Department Of Microbiology And Infectious Dise
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NAKAMURA Norimasa
McCaig Centre for Joint Injury and Arthritis Research, University of Calgary
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MARCHUK Linda
McCaig Centre for Joint Injury and Arthritis Research, University of Calgary
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Kaneda Yasufumi
Division Of Clinical Gene Therapy Osaka University Graduate School Of Medicine
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Frank Cyril
Mccaig Center For Joint Injury And Arthritis Research University Of Calgary
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Ota Naruhisa
Institute Of Applied Biochemistry University Of Tsukuba
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Taira Kazunari
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Yoshikawa Hideki
Department Of Applied Physics Osaka University
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Shrive Nigel
McCaig Centre for Joint Injury and Arthritis Research, University of Calgary
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Hart David
McCaig Centre for Joint Injury and Arthritis Research, University of Calgary
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