Induction of Apoptosis by Phosphatidylserine.
スポンサーリンク
概要
- 論文の詳細を見る
Treatment of Chinese hamster ovary (CHO) cells with phosphatidylserine (PS) caused cell death in a dose-dependent manner. Other phospholipids, such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidic acid, had no effect on cell viability. The cells incubated with PS became round and underwent a dramatic reduction of cellular volume while maintaining the membrane containment of cellular contents. The PS-treatment induced chromatin condensation and extensive DNA fragmentation, with a pattern characteristic of internucleosomal fragmentation on agarose gel electrophoresis. These results indicate that PS-treatment induces apoptosis of CHO cells. This apoptosis-inducing activity was highly specific for PS, and neither of the synthetic PS analogs 1, 2-diacyl-sn-glycero-3-phospho-D-serine (D-PS) and 2, 3-diacyl-sn-glycero-l-phospho-L-serine induced apoptosis. Analysis using fluorescence-labeled phospholipids showed that both PS and n-PS were taken up equally and then transported to intracellular membranes, suggesting that the PS-specific induction of apoptosis was not the result of its specific internalization. These observations suggest that certain molecules which may recognize the stereo-specific configuration of PS are involved in the apoptotie process triggered by PS.
- 社団法人 日本生化学会の論文
著者
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Umeda Masato
Department Of Functional Materials Science Saitama University
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Uchida Kayoko
Department Of Inflammation Research The Tokyo Metropolitan Institute Of Medical Science (rinshoken)
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Inoue Keizo
Department Of Biochemistry And Molecular Biology Indiana University
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Emoto Kazuo
Department Of Cell Biology Osaka Bioscience Institute
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Daleke David
Department of Biochemistry and Molecular Biology, Indiana University
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