Hepatic uptake of bilirubin diglucuronide: Analysis by using sinusoidal plasma membrane vesicles.
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概要
- 論文の詳細を見る
In order to characterize the mechanism for bilirubin transport in the liver, the uptake of bilirubin diglucuronide (BDG) into purified sinusoidal plasma membrane vesicles was investigated. BDG uptake was saturable, and was inhibited by sulfobromophthalein and unconjugated bilirubin, but was not affected by sodium taurocholate. BDG uptake was sodium-independent and was stimulated by intravesicular bilirubin or BDG (transstimulation). BDG transport showed strong potential sensitivity; vesicle inside-negative membrane potential created by different anion gradients inhibited BDG uptake whereas vesicle inside-positive membrane potential generated by potassium gradients and valinomycin markedly stimulated BDG transport. These data suggest that BDG, sulfo-bromophthalein, and probably unconjugated bilirubin share a common transporter in liver cells which is sodium independent, membrane-potential-dependent and capable of exchange. The direction of transport in vivo may be governed by the intracellular concentration of BDG and of other yet unidentified organic anions sharing this transporter.
- 社団法人 日本生化学会の論文
著者
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Adachi Yukihiko
Second Department Of Internal Medicine Kinki University School Of Medicine
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Kobayashi Hiroaki
Second Department Of Internal Medicine Sapporo Medical College
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Roy-Chowdhury Jayanta
Medicine, Albert Einstein College of Medicine
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Arias Irwin
Department of Physiology, Tufts University Medical School
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Roy-Chowdhury Namita
Medicine, Albert Einstein College of Medicine
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Kinne Rolf
Physiology, Albert Einstein College of Medicine
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Tran Thao
Medicine, Albert Einstein College of Medicine
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