A Ternary Conjugation System for the Construction of DNA Libraries for Geobacillus kaustophilus HTA426
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概要
- 論文の詳細を見る
This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.
- 公益社団法人 日本農芸化学会の論文
著者
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Doi Katsumi
Microbial Genetic Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University
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Ohshima Toshihisa
Microbial Genetic Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University
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DOI Katsumi
Microbial Genetic Division, Institute of Genetic Resources, Faculty of Agriculture, Graduate School, Kyushu University
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SUZUKI Hirokazu
Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu University
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WADA Keisuke
Applied Molecular Microbiology and Biomass Chemistry, Bioscience and Biotechnology, Graduate School, Kyushu University
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FURUKAWA Megumi
Functional Genomics of Extremophiles, Faculty of Agriculture, Graduate School, Kyushu University
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OHSHIMA Toshihisa
Microbial Genetic Division, Institute of Genetic Resources, Faculty of Agriculture, Graduate School, Kyushu University
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