Detection and Genotype of Encephalitozoon cuniculi DNA from Urine and Feces of Pet Rabbits in Japan
スポンサーリンク
概要
- 論文の詳細を見る
A newly developed nested polymerase chain reaction (PCR) assay targeting the internal transcribed spacer (ITS) region of the ribosomal DNA was applied to detect and characterize Encephalitozoon cuniculi DNA from pet rabbits in Japan. The analysis was carried out using 257 urinary samples and 314 fecal samples collected from 307 pet rabbits in the age group of 1 month to 12 years from 30 different prefectures of Japan and 107 fecal samples and 3 urinary samples collected from 1-month-old rabbits from 3 breeding facilities in Japan. We detected 840-bp amplicons in 20 urinary samples (7.78%) from the pet rabbits of the 13 prefectures and in 1 urinary (33.3%) and 6 fecal (5.6%) samples from the rabbits of the 2 breeding facilities. The sequences (803 bp) of the 27 amplicons had no variations and completely coincided with the sequence of E. cuniculi genotype I. To the best of our knowledge, this is the first report on the detection and genotype characterization of E. cuniculi DNA from pet rabbits in Japan.
- 公益社団法人 日本獣医学会の論文
著者
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Itagaki Tadashi
Laboratory Of Parasitology Department Of Disease Control And Environmental Sciences Faculty Of Agric
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Aoki Mikiko
Laboratory Of Parasitology Department Of Disease Control And Environmental Sciences Faculty Of Agric
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YAGITA Kenji
Department of Parasitology , National Institute of Health
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YAGITA Kenji
Department of Parasitology, National Institute of Infectious Diseases, 1–23–1 Toyama, Shinjuku, Tokyo 162–8640, Japan
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KIMURA Masami
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka 020–8550, Japan
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ICHIKAWA-SEKI Madoka
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka 020–8550, Japan
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MATSUO Kayoko
Pet Sun Co., Ltd., 1–5–3 Takamiya, Neyagawa, Osaka 572–0806, Japan
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ICHIKAWA-SEKI Madoka
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka 020–8550, Japan
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AOKI Mikiko
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka 020–8550, Japan
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