Morphological and Molecular Characterization of Explanatum explanatum from Cattle and Buffaloes in Myanmar
スポンサーリンク
概要
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A robust molecular marker is needed for discrimination of amphistome species, because identification based on morphology alone requires specialized knowledge and techniques. In this study, we performed morphological and molecular characterization of Explanatum explanatum, a species that causes severe liver damage in definitive host species. Fifty-five adult amphistomes were collected from cattle and water buffaloes in Myanmar. Eighteen of the amphistomes, arbitrarily chosen, were morphologically identified as E. explanatum using sagittal sections. All of the 55 amphistome isolates had identical second internal transcribed spacer (ITS2) of ribosomal DNA sequences; these sequences differed at 7 nucleotide sites from those of the closest species, Paramphistomum leydeni. Our data indicate that the ITS2 sequence could be a useful molecular marker for epidemiological studies on E. explanatum.
著者
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HTUN Lat
Department of Pharmacology and Parasitology, University of Veterinary Science
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MAW Ni
Livestock Breeding and Veterinary Department
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Katakura Ken
Laboratory Of Parasitology Department Of Disease Control Graduate School Of Veterinary Medicine Hokk
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Itagaki Tadashi
Laboratory Of Parasitology Department Of Disease Control And Environmental Sciences Faculty Of Agric
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SUNN Kyaw
Livestock Breeding and Veterinary Department
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BAWN Saw
Department of Pharmacology and Parasitology, University of Veterinary Science
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ICHIKAWA Madoka
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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KONDOH Daisuke
Department of Basic Veterinary Science, United Graduate School of Veterinary Science, Gifu University
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THEIN Myint
Livestock Breeding and Veterinary Department
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GYI Aung
Livestock Breeding and Veterinary Department
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KATAKURA Ken
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 8, Sapporo, Hokkaido 060–0818, Japan
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HTUN Lat
Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Naypyidaw 05282, Myanmar
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MAW Ni
Livestock Breeding and Veterinary Department, Insein, Yangon 11011, Myanmar
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