Ultrastructural Maturation of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in a Long-Term Culture
スポンサーリンク
概要
- 論文の詳細を見る
Background: In the short- to mid-term, cardiomyocytes generated from human-induced pluripotent stem cells (hiPSC-CMs) have been reported to be less mature than those of adult hearts. However, the maturation process in a long-term culture remains unknown. Methods and Results: A hiPSC clone generated from a healthy control was differentiated into CMs through embryoid body (EB) formation. The ultrastructural characteristics and gene expressions of spontaneously contracting EBs were analyzed through 1-year of culture after cardiac differentiation was initiated. The 14-day-old EBs contained a low number of myofibrils, which lacked alignment, and immature high-density Z-bands lacking A-, H-, I-, and M-bands. Through the long-term culture up to 180 days, the myofibrils became more tightly packed and formed parallel arrays accompanied by the appearance of mature Z-, A-, H-, and I-bands, but not M-bands. Notably, M-bands were finally detected in 360-day-old EBs. The expression levels of the M-band-specific genes in hiPSC-CMs remained lower in comparison with those in the adult heart. Immunocytochemistry indicated increasing number of MLC2v-positive/MLC2a-negative cells with decreasing number of MLC2v/MLC2a double-positive cells, indicating maturing of ventricular-type CMs. Conclusions: The structural maturation process of hiPSC-CMs through 1-year of culture revealed ultrastructural sarcomeric changes accompanied by delayed formation of M-bands. Our study provides new insight into the maturation process of hiPSC-CMs. (Circ J 2013; 77: 1307–1314)
著者
-
Kita Toru
Department Of Cardiology Kobe City Medical Center General Hospital
-
Kamakura Tsukasa
Department of Cardiology, National Cardiovascular Center
-
Yamanaka Shinya
Center For Ips Cell Research And Application (cira) Institute For Integrated Cell-material Sciences
-
Makiyama Takeru
Department Of Cardiology Hyogo Prefectural Amagasaki Hospital
-
Hattori Tetsuhisa
Department Of Cardiovascular Medicine Kyoto University
-
Horie Minoru
Department Of Cardiac Physiology National Cardiovascular Center Research Institute
-
Sasaki Kenichi
Department Of Cardiology Kitano Hospital Medical Research Institute
-
Ohno Seiko
Department Of Cardiovascular And Respiratory Medicine Shiga University Of Medical Science
-
KIMURA Takeshi
Department of Applied Chemistry and Molecular Science, Faculty of Engineering, Iwate University
-
Yamanaka Shinya
Center for iPS Cell Research and Application (CiRA), Institute for Integrated Cell-Material Sciences Kyoto University
-
Yoshida Yoshinori
Center for iPS Cell Research and Application (CiRA), Institute for Integrated Cell-Material Sciences Kyoto University
-
Wuriyanghai Yimin
Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine
-
Chen Jiarong
Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine
-
Kita Toru
Department of Cardiovascular Medicine, Kobe City General Hospital
-
Hattori Tetsuhisa
Department of Cardiovascular and Respiratory Medicine, Shiga University of Medical Science
関連論文
- N-Acetylcysteine Reduces the Severity of Atherosclerosis in Apolipoprotein E-Deficient Mice by Reducing Superoxide Production
- Incremental Effects of Eicosapentaenoic Acid on Cardiovascular Events in Statin-Treated Patients With Coronary Artery Disease : Secondary Prevention Analysis From JELIS
- PE-132 Induction of Vulnerable Plaques by Marble-Burying Behavior and Their Stabilization by Exercise Training in Experimental Atherosclerosis(PE023,Coronary Revascularization, PCI (DES) 3 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting
- FRS-059 Long-term Probucol Treatment Prevents Secondary Cardiovascular Events : A Cohort Study of Patients with Heterozygous Familial Hypercholesterolemia in Japan(FRS12,New Approaches to Atherosclerosis Research (IHD),Featured Research Session (English),
- Long-Term Probucol Treatment Prevents Secondary Cardiovascular Events : a Cohort Study of Patients with Heterozygous Familial Hypercholesterolemia in Japan
- PE-026 Swimming Reduces the Severity of Atherosclerosis in Apolipoprotein E Deficient Mice by Antioxidant Effects(Atherosclerosis, basic(03)(IHD),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Polymorphisms of Apolipoprotein E and Methylenetetrahydrofolate Reductase in the Japanese Population
- Exercise Training Reduces Severity of Atherosclerosis in Apolipoprotein E Knockout Mice via Nitric Oxide
- Effects of Obstructive Sleep Apnea with Intermittent Hypoxia on Platelet Aggregability
- PJ-577 The Antiplatelet Effect of Aspirin does not Change after 2-year Interval(PJ097,Cardiovascular Pharmacology (Clinical) (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- Clopidogrel Resistance in Japanese Patients Scheduled for Percutaneous Coronary Intervention(Ischemic Heart Disease)
- Evaluation of the Antiplatelet Effects of Cilostazol, a Phosphodiesterase 3 Inhibitor, by VASP Phosphorylation and Platelet Aggregation
- PJ-422 Platelet Aggregability under Aspirin Therapy in Smoker is Higher than That in Non-smoker(Cardiovascular pharmacology, basic/clinical(05)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-421 Anti-platelet Effects of Statins in Patients under Aspirin Therapies(Cardiovascular pharmacology, basic/clinical(05)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-031 Additional Gene Modifiers Reduce Effectiveness of β-blockers in the Long QT Type 1 Syndrome(Arrhythmia-Basic : Molecular and Genetic : Basis (A) : FRS4)(Featured Research Session (English))
- ECG Findings in Andersen's Syndrome : Action Potential Simulation Study
- Incidence of and Risk Factors for Contrast-Induced Nephropathy After Cardiac Catheterization in Japanese Patients
- Coronary Risk Factor Profile and Prognostic Factors for Young Japanese Patients Undergoing Coronary Revascularization
- PJ-795 Narrow QRS Duration Predicts Improvement of Left Ventricular Systolic Function by Beta-blockers in Chronic Heart Failure(PJ133,Heart Failure (Treatment) 4 (M),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation
- PJ-710 Efficasy of Catheter Ablation for Atial Fibrillation with Sinus Node Dysfunction(PJ119,Arrhythmia, Others (Clinical/Diagnosis/Treatment) 7 (A),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PJ-571 Multiple Mechanisms underlie Long QT Syndrome by the Change of Amino Acid R259 in the Intracellular S4-S5 Linker of KCNQ1(PJ096,Arrhythmia, Others (Clinical/Pathophysiology) 4 (A),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The
- PE-339 Survival after Coronary Artery Bypass Graft in Patients with Mitral Regurgitation(PE057,Myocardial Ischemia-reperfusion (Clinical) (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PE-106 SCN5A and Lamin A/C Gene Mutations are Highly Prevalent in Patients with Familial Bradyarrhythmic Disorders(PE018,Arrhythmia, Others (Clinical/Pathophysiology) (A),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulat
- Aldosterone Signaling Associates With p300/GATA4 Transcriptional Pathway During the Hypertrophic Response of Cardiomyocytes
- Magnitude of Sustained Multiple Risk Factors for Ischemic Heart Disease in Japanese Employees : A Case-Control Study
- PJ-795 Narrow QRS Duration Predicts Improvement of Left Ventricular Systolic Function by Beta-blockers in Chronic Heart Failure(PJ133,Heart Failure (Treatment) 4 (M),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation
- PJ-740 Comparison of Percutaneous Coronary Intervention (PCI) and Coronary Artery Bypass Graft Surgery (CABG) in Diabetic Patients (pts) : For the CREDO Investigators(Coronary revascularization, PCI-20, The 71st Annual Scientific Meeting of the Japanese C
- OE-392 Progranulin Expression in Advanced Human Atherosclerotic Plaque(OE66,Atherosclerosis (Clinical/Pathophysiology) (IHD),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- A Case Study on Cardiac Imaging in Patients with Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy : A Comparison between 64-Slice Computed Tomography, Magnetic Resonance Imaging and Electroanatomical Mapping
- Characterization of the Antiplatelet Effect of Aspirin at Enrollment and After 2-Year Follow-up in a Real Clinical Setting in Japan
- PJ-340 Curcumin, a Natural p300-specific Histone Acetyltransferase Inhibitor, Possesses Beneficial Effects in addition to ACE Inhibitor after Myocardial Infarction in Rats(PJ057,Translational Science (Cardiac) (H),Poster Session (Japanese),The 73rd Annual
- PE-528 Cyclin-dependent kinase-9 is Recruited on Cardiac Hypertrophic Response Gene Promoters through GATA4 in Cardiomyocytes(PE089,Cardiomyopathy/Hypertrophy (Basic) (M),Poster Session (English),The 73rd Annual Scientific Meeting of the Japanese Circulat
- PE-351 Synergistic Effects of G-CSF and Erythropoietin on Left Ventricular Systolic Function in Heart Failure after Rat Myocardial Infarction(PE059,Heart Failure (Treatment) 2 (M),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese
- PE-263 Neural Cell Adehesion Molecule is Upregulated by Metabolic Stress and Enhances the Cardiomyocyte Survival(PE044,Molecular Biology (M),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- FRS-064 NF-kB-dependent Induction of MicroRNA-146 Downregulates ErbB4 and may be Involved in the Deterioration of Cardiomyocyte Survival(FRS13,Novel Molecular Mechanisms of Heart Failure 2 (M),Featured Research Session (English),The 73rd Annual Scientific
- 2 Prognosis of Japanese Patients after Coronary Revascularization : Perspectives from CREDO-Kyoto Registry(Therapeutic Decision Making for Coronary Artery Disease Among PCI, CABG, and Medication,Symposium 3 (SY-03) (IHD),The 73rd Annual Scientific Meeting
- 3 Clopidogrel Resistance in Japanese Patients(Current Status and Future Perspective of Antiplatelet Therapy in Management of Coronary Artery Disease,Symposium 2 (SY-02) (I),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- A novel SCN5A gain-of-function mutation M1875T associated with familial atrial fibrillation
- Prevalence of Metabolic Syndrome in the General Japanese Population in 2000
- 2 Major Outcomes and Safety of EPA Derived from the Japan EPA LIPID INTERVENTION STUDY (JELIS)(Late Breaking Clinical Trials in Japan,The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Polymorphisms in Four Genes Related to Triglyceride and HDL-cholesterol Levels in the General Japanese Population in 2000
- Serum Lipid Survey and Its Recent Trend in the General Japanese Population in 2000
- PJ-904 Metabolomic Analysis of the Transition from Cardiac Hypertrophy to Failure(Metabolism / Biochemistry / Energetics(03)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-432 Chronic Apelin Administration Ameliorates Left Ventricular Dysfunction and Improves Survival in Hypertensive Rats with Heart Failure(Heart failure, basic-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-022 IGF Axis and Adiponectin as Prognostic Markers in Patients with Congestive Heart Failure(FRS5,Novel Biomarkers in Heart Failure (M),Featured Research Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- Serum Adiponectin Level as an Independent Predictor of Mortality in Patients With Congestive Heart Failure
- PJ-498 Serum Adiponectin Level Predicts Mortality And Inversely Correlates to Insulin-like Growth Factor Axis in Patients with Congestive Heart Failure(Heart failure, clinical-14, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-281 The Impact of Response to Cardiac Resynchronization Therapy on Ventricular Arrhythmic Events(OE48,CRT (M),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- OE-162 The Impact of CYP2C19 Polymorphism on the Antiplatelet Effect of Clopidogrel in the Real Clinical Setting(OE28,Coronary Revascularization, PCI (Restenosis/Others) 1 (IHD),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanes
- OE-031 Visualization of Accumulating Reactive Oxygen Species during Myocardial Ischemia by Real-time Two-photon Imaging of Perfused Rat Hearts(OE06,Myocardial Ischemia-reperfusion (Basic) (IHD),Oral Presentation (English),The 73rd Annual Scientific Meetin
- PJ-272 Cyclin-dependent kinases-9 is required for the differentiation of mouse ES cells into cardiac myocytes.(Development and differentiation(02)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OE-334 Overexpression of MicroRNA-1 in Mouse Embryonic Stem Cells Represses Myocardial Differentiation(Development and differentiation(01)(M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OE-233 MicroRNA-133 Regulates the Expression of GLUT4 by Targeting KLF15 and is Involved in Metabolic Control in Cardiac Myocytes(Metabolism/Biochemistry/Energetics(01)(M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circu
- OE-166 Upregulation of MicroRNA Targeting Retinoid X Receptor A Contributes to Development of Cardiac Hypertrophy(Molecular biology, myocardium (M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-760 Cyclin-Dependent Kinases-9, as a Novel Component of p300/GATA-4 Complex, is Involved in Differentiation of Mouse ES Cells into Cardiac Myocytes(Development and differentiation-2, The 71st Annual Scientific Meeting of the Japanese Circulation Societ
- PE-531 A GATA-4 Mutant Lacking p300-Induced Acetylation Suppresses Myocardial Cell Hypertrophy(Molecular biology, myocardium-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-419 Enkephalin is Required for Adipocyte Differentiation and is Involved in Impaired Glucose Tolerance in Rat Model of Type II Diabetes(Diabetes/Obesity/Metabolic syndrome-06, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-417 Activation of TG-interacting Factor by Insulin Regulates Adipocyte Differentiation(Diabetes/Obesity/Metabolic syndrome-06, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-409 Functional Cloning of the Genes which Regulates GLUT4 Trafficking in Cardiac Myocytes(Diabetes/Obesity/Metabolic syndrome-05, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-172 miR-133 Controls GLUT4 Expression by Targeting KLF15 in Cardiomyocytes(Metabolism/Biochemistry/Energetics-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-171 Oxidative Stress Induces GLUT4 Translocation by Dual AMPK Kinase Activation in Cardiac Myocytes(Metabolism/Biochemistry/Energetics-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-075 Suppression of Insulin Signaling Attenuates Cardiac Aging in Mice(Heart failure, basic(01)(M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-533 CCN1 Protects Cardiac Myocytes from Oxidative Stress via β 1 Integrin-Akt Pathway(Molecular biology, myocardium-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Sirolimus-Eluting Stent for In-Stent Restenosis of Left Main Coronary Artery in Takayasu Arteritis
- Clinical Experience with Gunther Tulip Retrievable Vena Caval Filter for Prevention of Pulmonary Embolism : Retrieval Is Easy and Safety (Thrombosis/Thrombolysis 1 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Chromatin Remodeling during Differentiation of ES Cells into Cardiac Myocytes(Cardiovascular Development, The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Combined Measurements of Cardiac Troponin T and N-Terminal Pro-Brain Natriuretic Peptide in Patients With Heart Failure
- PJ-464 Delayed Relaxation Detected by Regional Contraction Curve of Real-Time 3D Echocardiography Accurately Reflects Local Ischemia(Angina Pectoris, Clinical 2 (IHD) : PJ77)(Poster Session (Japanese))
- PE-462 Real-Time 3D Echocardiography Provides an Index of LV Diastolic Property : max dV/dt(Cardiac Function, Basic/Clinical 3 (M) : PE79)(Poster Session (English))
- OE-363 Activation of cardiac aldosterone system induces association of mineralocorticoid receptor with transcriptional coactivator p300 during hypertrophic responses in cardiac myocytes(Cardiac Hypertrophy, Basic and Clinical 1 (M) : OE46)(Oral Presentati
- OE-362 Lys-CoA, a Specific Inhibitor of p300-Mediated Acetylation, Represses Hypertrophic Responses in Cardiac Myocytes(Cardiac Hypertrophy, Basic and Clinical 1 (M) : OE46)(Oral Presentation (English))
- A Specific Inhibitor of Histone Deacetylase, Trichostatin A, Promotes Differentiation of Embryonic Stem Cells into Cardiac Myocytes
- Inhibition of Aldosterone Suppresses PDGF-and Basic FGF-Induced Proliferative Change in Vascular Smooth Muscle Cells
- Overexpression of P300 Promotes Left Ventricular Endothelin-1 Expression and Remodeling Following Myocardial Infarction in Adult Mice in Vivo
- Inhibition of Cardiac Aldosterone System by Spironolactone Blocks Gq Protein Coupled Agonist-Induced Hypertrophic Responses in Cardiac Myocytes
- A Transcriptional Coactivator P300 Associates with Calcineurin-Dependent Signaling during the Transcription of Bcl-2 Gene in Cardiac Myocyte
- Cardiac Nuclear Hyper-Acetylation by Inhibiting Histone Deacetylase Facilitates Left Ventricular Remodeling Following Myocardial Infarction In Adult Mice in Vivo
- OE-317 MicroRNA-206 Controls Expression of α-and β-Myosin Heavy Chain Genes in Cardiac Myocytes(Molecular biology, myocardium-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-109 Trapping of Genes that Encode Secreted and Transmembrane Proteins in H9C2 cardiomyoblasts(Growth factor/Cytokine-1(H/M), The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-024 Up-Regulated Expression of MicroRNA-143 in the Adipose Tissue of High-Fat Diet-Induced Obese Mice(Diabetes/Obesity (basic), The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-349 Leptin Induces Physiological and Compensated Growth of the Left Ventricles Following Myocardial Infarction in Vivo(Diabetes/Obesity/Metabolic syndrome-7 (H) PE59,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanes
- PE-245 Adipocyte-derived Serotonin Regulates Adipocyte Differentiation via an Autocrine/paracrine Mechanism(Diabetes/Obesity/Metabolic syndrome-8 (H) PE41,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation
- PE-103 The Role of cAMP/PKA Pathway in the Differentiation of Embryonic Stem Cells into Cardiac Myocytes(Development and differentiation-2 (M) PE18,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society
- PJ-501 Serum Soluble Lectin-like Oxidized Low-density Lipoprotein Receptor-1 Levels are Elevated in Congestive Heart Failure(Heart failure, clinical-15, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Clinical Features of Familial Hypercholesterolemia in Japan in a Database from 1996-1998 by the Research Committee of the Ministry of Health, Labour and Welfare of Japan
- Mutations in Japanese Subjects with Primary Hyperlipidemia : Results from the Research Committee of the Ministry of Health and Welfare of Japan since 1996
- OJ-084 Risk of Ventricular Tachyarrhythmias Predicted by Biochemical Markers Associated with Cardiac Remodeling(Heart failure, clinical-05, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Correlation between Cardiac TroponinT and the Other Biochemical Markers in Patients with Myocyte Injury(Heart Failure, Clinical 18 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- PE-124 Real-Time 3D Echocardiography is a New Modality that Provides Accurate LV Volumetry : Comparisons with Conventional 2D-Echo Measurements(Echo/Doppler 5 (I) : PE21)(Poster Session (English))
- OJ-272 Quantitative Analysis of Regional Ventricular Motion by Real-Time 3D Echocardiography : Comparative Study to Semi-Quantifications by Conventional 2D-Echo Measurements and LVG(Echo/Doppler 10 (I) : OJ32)(Oral Presentation (Japanese))
- OE-091 Detection of cardiac sarcoidosis with delayed-enhanced MRI : Comparison with nuclear imaging(X-ray/CT/MRI/DSA 1 (I) : OE11)(Oral Presentation (English))
- 3 Prevalence and Significance of Risk Factors in Japanese Patients Undergoing Coronary Revascularization : from CREDO-Kyoto Registry(Comprehensive Risk Management for Prevention of Coronary Artery Disease,Symposium 16 (SY-16) (H),The 73rd Annual Scientifi
- Myocardial Regulation of p300 and p53 by Doxorubicin Involves Ubiquitin Pathways
- OE-257 Cyclin-dependent Kinase-9 is Required for Histone Acetyltransferase Activity of p300(Cardiac hypertrophy, basic/clinical(01)(M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- 1 A non-toxic dietary compound curcumin provides a novel heart failure therapy that targets nuclear signaling pathway in cardiac myocytes(Symposium 7 (SY-07) (M) Molecular Mechanism and Novel Therapeutic Target in Heart Failure,Special Program,The 72nd An
- PE-532 Proteomics Analysis Identifies Novel GATA-4-binding Partners Involved in Hypertrophic Responses in Cardiac Myocytes(Molecular biology, myocardium-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-435 A Natural p300-specific Histone Acetyltransferase Inhibitor, Crucumin, Prevents the Development of Hypertension-induced Heart Failure in salt-sensitive Dahl Rats(Heart failure, basic-4, The 71st Annual Scientific Meeting of the Japanese Circulation
- 1 A Novel Genetic Marker for Potential Risk of QT Prolongation and Cardiac Sudden Death : KCNE1-D85N Polymorphism(Biomarkers, Genetic Polymorphism, and Risk Estimation,Symposium 1 (SY-01) (H),The 73rd Annual Scientific Meeting of The Japanese Circulation
- Mutation Analysis of the Glycerol-3 Phosphate Dehydrogenase-1 Like (GPD1L) Gene in Japanese Patients With Brugada Syndrome
- A Novel Mutation Associated With Jervell and Lange-Nielsen Syndrome in a Japanese Family