Serum Cobalt Reaction as a Liver Function Test and Immunoglobulin

概要

論文の詳細を見る
The procedure of serum cobalt reaction by Inoue et al. 1) is as follows: 0.1 ml of serum is added to each of 10 tubes, with 1.2 mg, 1.0mg, -and 0.3mg of CoC12·6H20/ 5ml, called as R1, R2, -and R10, respectively. They are incubated for 15 minutes in boiling water bath. The highest number of the tube, in which coagulated sediments are found, is adopted for the cobalt value. Of 267 in-patients, the cobalt value were as follows: 88% of 100 cases with cancer showed R0-3. 86% of 37 cases with acute infection showed R0-3. 81% of 50 cases with liver cirrhosis showed R6-10. Of 80 cases with the other diseases, 91% showed R2-5. In addition to the foregoing report 2) , the present studies are undertaken in a attempt to elucidate what in the serum of the patients with those diseases induces an abnormal value of the cobalt reaction. Now the cobalt value of some serum gave R5. The only high molecular parts, isolated from the serum by Sephadex G-50 column chromatography, showed R8 in spite of containing an equivalent amount of protein to that in the original system. R8 came back to R5 by supply of low molecular parts with an equivalent amount of phosphorus. This fact indicates that the cobalt reaction is composed of both of these parts. So, which of the high molecular parts of pathologic serum or the low ones takes part in the reaction was examined, and it was revealed as a result that the reaction depended upon the formers. However, no effect was found by the use of the serum containing 6. 6-7. 9g/dl of protein. The quality is the question. By DEAE Sephadex A-50 column chromatography, the high molecular parts were fractionated to four pieces; each protein of which corresponds to γ-globulin, α2+β-globulin, alubumin and α1-globulin, respectively, and these fractions were added to the original system after dialysis in order to check the effect. The result was that an increase by Fr 1 and a decrease by Fr 2 or 4 were reciprocally induced. No effect by Fr 3 appeared. Difference in strength of the effect among the materials was found only in Fr 1. The protein of Fr 1, which was identified to be the IgG itself caught from the serum with a yield of 96.6%, were further fractionated to A, B, C and D by CM Sephadex C-50 column chromatography. The amounts in average of the 8 cases were as follows: A-0.9, B-0.5, C-7.4, D-2.8mg/ml, C/D-2.5 (normals). A-1. 1, B-0.5, C-18. 9, D-6.2mg/ml, C/D-3.0 (cirrhotics).It was C IgG that was related with the cobalt reaction. Of interest in this connection is the fact that the clinical cases, with an increase mainly in D IgG, showed a relatively normal cobalt value. C IgG was different from D IgG in electrophoretic property, amino acid analysis, and such biological features as changes of the amounts related with age and high sensibility for glucocorticoid, but not in sedimentation coefficient and immunological nature. By means of the Fleischman's procedure 5) , C and D IgG were disso ciated respectively into light and heavy chains. Of these components, the light chains of both could not be distinguished in immunological properties from Bence-Jones protein, independently of K or L type, but Lchain of C IgG only increased the cobalt value. These findings lead us to the interesting conclusion that some reactive protein in the serum cobalt reaction and Bence-Jones protein in myeloma urine-both revealed the specific feature against heat-were identical in respect to the light chain of IgG.
Japan Society of Clinical Chemistryの論文

Serum Cobalt Reaction as a Liver Function Test and Immunoglobulin

スポンサーリンク

概要

著者

関連論文

スポンサーリンク