A New Kunkel Reagent Using Bis (2- hydroxyethyl) iminotris (hydroxymethyl) methane (Bis-Tris) Buffer

スポンサーリンク

概要

• 論文の詳細を見る

• The zinc sulfate turbidity test (ZTT) is a convenient assay procedure to follow qualitative and quantitative changes in serum proteins, and it has been used clinically as an important di-agnostic test. The standard procedure of ZTT uses a Kunkel reagent which is a solution of zinc sulfate in 2.6mM barbiturate buffer. However, it is difficult for this buffer to maintain its pH in solution which is important for the test. The pH in the solution decreases due to absorption of carbon dioxide from the air when the vial is opened and due to instability of barbiturate even when the vial is capped. As a result, the sensitivity increases with use leading to variation between assays and between laboratories. For these reasons, we developed a new Kunkel reagent using a Bis-Tris buffer which has a low dissociation constant and is chemically stable. The results using the new Kunkel reagent correlated with those using the standard Kunkel reagent, and the former showed good stability even when the vial was opened to the air. This new reagent may contribute to renewed interest in the clinical significance of ZTT.

• Japan Society of Clinical Chemistryの論文

著者

• 徳田 邦明

  和光純薬工業

• 濱田 恵子

  和光純薬工業株式会社大阪研究所

関連論文

• 集団検診における尿比重測定の意義
• 随時尿による定量値の各種補正効果 : 第2報 腎疾患患者尿での補正効果
• 過酸化物質測定用酸化還元呈色試薬
• A New Kinetic Method for Serum γ-Glutamyltransferase Activity Using L-γ-Glutamy1-3-Hydroxymethyl-4-Nitroanilide
• A New Method of Measuring Bilirubin in Serum by Vanadic Acid
• A New Kunkel Reagent Using Bis (2- hydroxyethyl) iminotris (hydroxymethyl) methane (Bis-Tris) Buffer

スポンサーリンク