A New Method of Measuring Bilirubin in Serum by Vanadic Acid

概要

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A new method for measuring bilirubin using vanadic acid as oxidizing agent was studied. A solution of a cation surfactant, cetyltrimethylammonium bromide (CTAB) as the reaction accelerator and that of a reducing agent, hydroxylamine as the reaction inhibitor in citrate or tartarate buffer, pH 3 (the first reagent) were used for measuring the concentration of total bilirubin and direct bilirubin, respectively. A solution of vanadic acid (4 mmol/l) containing ethylenediamine tetraacetic acid (EDTA) or hydroxyethan-di-phosphonic acid, pH 7 was used as the second reagent. The bilirubin concentrations were calculated by a two point method measuring absorbances at a specific wavelength before and after oxidation reaction. Under these experimental conditions, the oxidation reaction was accomplished within 2 to 3 minutes.<BR>This new method had fundamental abilities for the determination, such as an appropriate dynamic range, a high reproducibility and the good correlation with the diazo method. Furthermore, the results were affected very little by coexisting substances in serum.<BR>In addition, such liquid type reagents are very convenient without being prepared for every determination and will contribute to the correction of the day-to-day differences and save works in the laboratories.
Japan Society of Clinical Chemistryの論文