Identification of ALP-Linked Lmmmoglobulin by Immunoprecipitin Reaction in Free Liquid Media
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Enzyme-immunoelectrophoresis and enzyme-immmofixation on agar-agarose gel or cellogel membrane are usually used for the identification of alkaline phosphatase (ALP)-linked immunoglobulin (lg).However, there are some cases which could not be detected by these methods.<BR>We applied the immunoprecipitin reaction in free liquid media for the sensitive method of the identification of ALP-linked lg. By this method, it is easy to determine existence of ALP-linked lg from ALP activity in immunoprecipitate formed by each monospecific antiserum. ln the presernt study, ALP-linked lgG (λ) was identlfied in all of 6 cas es by this method. The result corresponded with that from immunoelectrophoresis.Besides, we observed the change of ALP-linked lgG in clinical course and could confirm the sensitive method, by which ALP-lhked lgG in every case could be detected even that could not be detected by enzyme-immunoe-ectrophoresis.<BR>High sensitivity of this method for the detection of ALP-linked lgG comes from that many precipitates were obtained and from that procedure time was short enough to avoid loss of ALP activity.<BR>As ALP bound to lgG in almost cases, we also tested the method using Protein A-Sepha rose CL-4B.However its detective sensitivity was most, ALP-linked lgG3, lgA and lgM could not be identified.Therefore, the immunoprecipitin reaction was demonstrated as the simple, quick and reliable method to identify both class and type of ALP-lhked lg.
- Japan Society of Clinical Chemistryの論文
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- Identification of ALP-Linked Lmmmoglobulin by Immunoprecipitin Reaction in Free Liquid Media